水稻种子耐储藏性状QTLs挖掘及候选基因分析

QTLs mapping and expression analysis of candidate genesfor root traits in rice

为挖掘水稻种子耐储藏性状相关基因,分别以华占(HZ)/热研(Nekken2)和天丰(TF)/热研(Nekken2)为亲本杂交产生F 1代,连续自交多代后得到的2套重组自交系(recombination inbred lines,RILs)群体为实验材料,统计2套群体分别储藏1年和2年的发芽率及其比率数据.利用实验室前期已构建好的分子连锁图谱,对水稻种子耐储藏性状的QTLs进行初步定位分析.结果表明:在HZ-RIL中检测到10个QTLs,分布于水稻第1,2,4,6,8和10号染色体上,其中位于8号染色体上的1个QTL位点LOD值高达5.48;在TF-RIL中同样也检测到10个QTLs,分别位于水稻第2,4,8,9,10和11号染色体上;对检测到的QTLs区间进行候选基因的筛选和分析,共筛选到可能与水稻耐储藏性状相关的11个候选基因.以上结果为挖掘新的水稻耐储藏基因及分子育种实践提供了物质基础,对培育水稻耐储藏优良品种具有重要意义.

In order to screen out the genes related to rice seed storage tolerance,Huazhan(HZ)/Nekken2(Nekken2)and Tianfeng(TF)/Nekken2(Nekken2)were used as parents to cross to produce F1 generation,and two sets of recombinant inbred lines(RILs)populations obtained after continuous self crossing for several generations were used as experimental materials to count the germination rate and its ratio data of the two sets of populations in different years.Based on the molecular linkage map constructed in the laboratory,the QTLs of rice seed storage tolerance was preliminarily located and analyzed.The results showed that 10 QTLs were detected in Huazhan RIL,which were distributed on chromosomes 1,2,4,6,8 and 10 of rice.The LOD value of one QTL site on chromosome 8 was as high as 5.48;Ten QTLs were also detected in Tianfeng RIL,located respectively on chromosome 2,4,8,9,10 and 11 of rice;At the same time,screening and analyzing the candidate genes in the detected QTLs intervals,11 candidate genes possibly related to the storage tolerance traits of rice were screened.The experimental results would provide a material basis for screening new rice storage tolerance genes and molecular breeding practice,and they would be of great significance for breeding rice varieties with good storage tolerance.