我国入侵害虫西部喙缘蝽的溯源分析

Tracking the invasive pest Leptoglossus occidentalis Heidemann,1910 in China

本文对我国外来入侵物种西部喙缘蝽Leptoglossus occidentalis Heidemann,1910进行了溯源分析,依据对国内种群的观察和标本简要描述了其形态特征和生活习性。通过野外采集国内种群标本,提取扩增COI基因序列,结合原产地和国外其他入侵种群的公共序列,构建西部喙缘蝽COI基因数据集,以中国入境口岸对木材的检疫情况为参考,分析西部喙缘蝽的入侵来源。全世界目前已知10种单倍型,中国存在4种。山东青岛种群有H_8、H_9和H_10三种单倍型,其中H_8与韩国共享,H_9和H_10为中国特有单倍型,青岛种群可能部分来自韩国;山东烟台两个采集点的种群都属于H_2,可能来自北美东部,与青岛种群的入侵来源不同。以上分析表明,我国西部喙缘蝽种群可能来源于多次独立入侵事件,同时在国内发生了小范围的扩散迁移。本研究首次对我国入侵害虫西部喙缘蝽做了溯源分析,为更好地制定规制措施,开展检疫防治工作提供了科学依据。

This study conducted an analysis on tracking the invasive species Leptoglossus occidentalis Heidemann,1910 in China.Based on the observations and specimens of the invasive population,the morphological characteristics and the biology of the species were briefly summarized.By extracting and amplifying COI gene sequences from the invasive population,a COI gene dataset for L.occidentalis was constructed by combining with public sequences from the original and other foreign invasive populations.The invasion sources of L.occidentalis were analyzed by reference to the quarantine situation of wood at China's entry ports.Currently,10 haplotypes are known worldwide,including 4 in China.In Shandong Province,the Qingdao population has three haplotypes,H_8 was shared with South Korea,while H_9 and H_10 were restricted in China.The Qingdao population might had originated from South Korea.The Yantai populations collected from two different sites in Shandong Province both belonged to H_2,which might had originated from eastern North America,which was different from that of the Qingdao populations.The above analysis indicated that the L.occidentalis populations in China might had originated from multiple independent invasion events,while small-scale dispersal occurred within the country.This study made the first tracking analysis of L.occidentalis in China,which provided scientific evidences for the formulation of regulatory measures and the implementation of quarantine and control.