火龙果茎多糖对调节小鼠细胞免疫功能的研究及毒性评价

Toxicity Evaluation of Polysaccharides from Pitaya Stem on Regula-ting Cellular Immune Function in Mice

为了探究火龙果茎多糖(PSP)对环磷酰胺所致免疫低下小鼠细胞免疫功能的调节作用,本研究对火龙果茎多糖进行毒性评价,通过检测小鼠淋巴细胞在有丝分裂原诱导下的增殖能力,计算小鼠体重、脾指数、全脾免疫计数以及血细胞计数等来评价小鼠细胞的免疫功能。结果表明:PSP对淋巴细胞存活率无抑制作用,PSP-1和5倍浓度条件下的PSP-2对T淋巴细胞有相同的增殖效应,对B淋巴细胞无明显影响;在免疫低下小鼠模型中,经PSP处理的小鼠脾指数和血细胞计数均有所缓解,且高剂量组效果较好,而对全脾免疫细胞的提升不明显。本研究表明,PSP-2可能是PSP-1中发挥免疫功能的活性组成成分,T淋巴细胞为主要效应细胞,火龙果茎多糖发挥免疫功能主要是通过促进淋巴细胞增殖能力来实现的。

The aim of this study was to investigate the effects of pitaya stem polysaccharide(PSP)on the cellular im-mune function in mice with cyclophosphamide induced immunodeficiency.In this study,the toxicity of pitaya stem polysaccharide was evaluated,and the immune function of mouse cells was evaluated by detecting the proliferation ability of mouse lymphocytes induced by mitogen,calculating the body weight,spleen index,spleen immune count and blood cell count of mice.PSP had no inhibitory effect on the survival rate of lymphocytes.PSP-1 and PSP-2 had the same proliferation effect on T lymphocytes,but had no obvious effect on B lymphocytes.In the immunocompromised mouse model,the splenic index and blood cell count of mice treated with PSP were alleviated,and the effect was better in the high-dose group,but the enhancement of whole splenic immune cells was not obvious.The results showed that PSP-2 may be the active component of PSP-1 to exert immune function,T lymphocytes are the main effector cells,and the immune function of dragon fruit stem polysaccharide is mainly achieved by promoting lymphocyte proliferation.