ABCG2及microRNA基因多态性与鼻咽癌易感性的关系

Relationship between ABCG2 and microRNA gene polymorphism and susceptibility to nasopharyngeal carcinoma

目的:探讨ABCG2及microRNA基因多态性与鼻咽癌易感性的关系。方法:2019年01月至2023年01月间我院肿瘤科住院的102例鼻咽癌患者作为研究对象,通过收集相关临床资料、采集鼻咽癌患者及健康人外周血并进行ABCG2基因、hsa-miR-499及hsa-miR-608基因多态性分析。结果:miR499:rs3746444(T→C)和miR608:rs4919510(C→G)在研究组和对照组之间存在差异(P<0.05)。通过风险因素分析,证实以rs3746444TT对照,rs3746444C变异基因型(TC+CC)个体发生鼻咽癌的危险增加45%(OR=1.45,95%CI=1.05~1.78),携带miR608:rs4919510(C→G)GC+GG的基因发生鼻咽癌是携带CC基因的1.53倍,(OR=1.53,95%CI=1.02~2.26);总和分析ABCG2 G34A基因GA+AA在鼻咽癌组的分布频率为57.84%,与对照组相比(32.00%)明显增高(OR=1.20,95%CI:1.02~1.42)。如果等位基因由G突变为A,发病风险亦明显提高(OR=1.18,95%CI:1.03~1.35);ABCG2 C421A等位基因由C突变为A,鼻咽癌发病风险亦明显增高(OR=1.12,95%CI:1.02~1.29)。ABCG2 G34A、ABCG2 C421A使鼻咽癌发病风险明显增高;2年患者生存率91.18%(93/102),患者性别、年龄、分化程度、吸烟、饮酒、EBV病毒感染及家族遗传史无差异(P>0.05),与TNM分期、淋巴结转移、ABCG2 G34A、ABCG2 C421A、miR499:rs3746444(T→C)及miR608:rs4919510(C→G)存在差异(P<0.05);miR499+G34A+C421A+miR608对鼻咽癌诊断价值最高。结论:ABCG2、miR499及miR608多态性与鼻咽癌易感性相关,影响患者预后,对鼻咽癌有一定的诊断价值。

Objective:To investigate the relationship between ABCG2 and microRNA gene polymorphism and susceptibility to nasopharyngeal carcinoma.Methods:102 in patients with nasopharyngeal carcinoma in the department of oncology of our hospital from January 2019 to January 2023 were collected as the study subjects,and the polymorphism of ABCG2 gene,hsa-miR-499 and hsa-miR-608 gene were analyzed by collecting relevant clinical data and peripheral blood of patients with nasopharyngeal carcinoma and healthy subjects.Results:miR499:RS3746444(T→C)and miR608:RS4919510(C→G)showed significant differences between the study group and the control group(P<0.05).According to the risk factor analysis,compared to rs3746444TT,rs3746444C variant genotype(TC+CC)individuals had a 45%increased risk of nasopharyngeal carcinoma(OR=1.45,95%CI=1.05~1.78).The incidence of nasopharyngeal carcinoma in miR608:RS4919510(C→G)GC+GG gene was 1.53 times of CC gene(OR=1.53,95%CI=1.02~2.26).The distribution frequency of ABCG2 G34A gene GA+AA in nasopharyngeal carcinoma group was 57.84%,significantly higher than that in control group(32.00%)(OR=1.20,95%CI:1.02~1.42).If the allele was mutated from G to A,the risk was significantly increased(OR=1.18,95%CI:1.03~1.35).The mutation of ABCG2 C421A allele from C to A significantly increased the risk of nasopharyngeal carcinoma(OR=1.12,95%CI:1.02~1.29).ABCG2 G34A,ABCG2 C421A A significantly increased the risk of nasopharyngeal carcinoma.The 2-year survival rate was 91.18%(93/102),and there were no differences in gender,age,degree of differentiation,smoking,alcohol consumption,EBV infection and family genetic history(P>0.05).There were differences in TNM staging,lymph node metastasis,ABCG2 G34A,ABCG2 C421A,miR499:rs3746444(T→C)and miR608:rs4919510(C→G)(P<0.05).miR499+G34A+C421A+miR608 had the highest diagnostic value for nasopharyngeal carcinoma.Conclusion:ABCG2,miR499 and miR608 polymorphisms are associated with susceptibility to nasopharyngeal carcinoma,which are prognostic factors and have certain diagnostic value for nasopharyngeal carcinoma.