鸦胆子油乳注射液可能通过调节JAK2/STAT3信号通路抑制胰腺癌AsPC-1细胞上皮-间质转化

Impacts of Brucea Javanica Oil Emulsion Injection on EpithelialMesenchymal Transition of Pancreatic Cancer AsPC-1 Cells by RegulatingJAK2/STAT3 Signaling Pathway

该文旨在探讨鸦胆子油乳注射液(简称鸦胆子油乳)调节JAK2/STAT3信号通路对胰腺癌细胞上皮-间质转化(EMT)的影响。将胰腺癌AsPC-1细胞分为对照组、鸦胆子油乳低剂量组、鸦胆子油乳中剂量组、鸦胆子油乳高剂量组、鸦胆子油乳高剂量+Colivelin(JAK2/STAT3激活剂)组。该研究采用细胞划痕实验检测细胞迁移;Transwell实验检测细胞侵袭;MTT、EdU检测细胞增殖;流式细胞术检测细胞凋亡率;qRT-PCR检测细胞JAK2 mRNA、STAT3 mRNA表达;Western blot检测细胞JAK2/STAT3通路、E-cadherin、N-cadherin、Vimentin蛋白表达水平。结果显示,与对照组相比,鸦胆子油乳低、中、高剂量组细胞活力、增殖率、细胞划痕愈合率、侵袭细胞个数、JAK2mRNA、STAT3 mRNA、N-cadherin、Vimentin、p-JAK2/JAK2、p-STAT3/STAT3表达水平显著降低,细胞凋亡率和细胞E-cadherin表达水平显著升高(P<0.05)。与鸦胆子油乳高剂量组相比,鸦胆子油乳高剂量+Colivelin组细胞活力和增殖率、细胞划痕愈合率与侵袭细胞个数、JAK2 mRNA、STAT3 mRNA基因表达水平、N-cadherin、Vimentin、p-JAK2/JAK2、p-STAT3/STAT3蛋白表达水平显著升高,细胞凋亡率和E-cadherin表达水平显著下降(P<0.05)。该研究得出鸦胆子油乳可以抑制AsPC-1细胞增殖、迁移、侵袭和EMT,这可能是通过抑制JAK2/STAT3信号通路实现的。

The aim of this study was to investigate the impacts of Brucea javanica oil emulsion injection on EMT(epithelial-mesenchymal transition)of pancreatic cancer cells by regulating JAK2/STAT3 signalingpathway.AsPC-1 cells from pancreatic cancer were grouped into control group,low dose group,medium dosegroup,high dose group,and high dose+Colivelin(JAK2/STAT3 activator)group.Cell scratch test was applied todetect cell migration;Transwell experiment was applied to detect cell invasion;MTT and EdU were applied todetect cell proliferation;flow cytometry was applied to detect cell apoptosis rate;qRT-PCR was applied to detectthe expression of JAK2 mRNA and STAT3 mRNA in cells;Western blot was applied to detect the expression levels of JAK2/STAT3 pathway,E-cadherin,N-cadherin,and Vimentin proteins in cells.Result display,compared with the control group,the cell viability and proliferation rate,scratch healing rate,the number of invasive cells,the expression of JAK2 mRNA,STAT3 mRNA,N-cadherin,Vimentin,p-JAK2/JAK2,p-STAT3/STAT3 proteinsin the low,medium,and high dose Brucea javanica oil emulsion groups obviously reduced,the apoptosis rateand the expression of E-cadherin in cells obviously increased(P<0.05).Compared with the high-dose Bruceajavanica oil emulsion group,the cell viability and proliferation rate,scratch healing rate,the number of invasivecells,thew expression of JAK2 mRNA,STAT3 mRNA,N-cadherin,Vimentin,p-JAK2/JAK2,p-STAT3/STAT3proteins in the high-dose Brucea javanica oil emulsion+Colivelin group obviously increased,the apoptosis rateand the expression of E-cadherin in cells obviously decreased(P<0.05).The study concluded that Brucea javanica oil emulsion can reduce AsPC-1 cell proliferation,migration,invasion,and EMT,possibly by inhibiting theJAK2/STAT3 signaling pathway.