佤药娘母良药酒通过抑制凋亡对勃起功能障碍大鼠的机制研究

Mechanistic Study of Niang-Mu-Liang Medicated Wine,A Traditional Wa Medicine,in Inhibiting Apoptosis in Rats with Erectile Dysfunction

目的研究佤药娘母良药酒对勃起功能障碍(ED)大鼠的治疗作用,及对凋亡信号通路的影响。方法将60只SD大鼠随机平均分为假手术、模型、白酒、西地那非、娘母良低剂量、娘母良高剂量6组。通过双侧髂内动脉结扎法建立ED模型。各组给药均持续28 d,给药结束后进行APO实验,测定各组大鼠睾丸和附睾的质量和脏器指数、精子浓度以及精子活率。对各组大鼠阴茎海绵体与睾丸组织进行HE染色。采用生化试剂盒和ELISA检测各组大鼠阴茎海绵体组织中NO和cGMP含量,采用Western blot检测凋亡信号通路相关蛋白的表达。结果与模型组相比,西地那非与娘母良干预后ED大鼠勃起次数、睾丸与附睾的质量和指数、精子数量及存活率均显著升高,阴茎海绵体与睾丸组织病理形态明显改善,阴茎海绵体组织NO以及cGMP含量升高,Bax/Bcl-2,Cleaved-caspase3和Cleaved-caspase9蛋白表达下降。结论娘母良药酒可以促进NO与cGMP的产生并抑制阴茎海绵体组织细胞凋亡进而改善ED大鼠勃起功能。

Objective To investigate the therapeutic effects of the traditional Wa medicine niang-mu-liang medicated wine on erectile dysfunction(ED)in rats and its impact on apoptotic signaling pathway.Methods A total of 60 SD rats were randomly divided into six groups:sham,model,white wine,sildenafil,niang-mu-liang low-dose,and niang-mu-liang highdose.ED model was established using bilateral ligation of the internal iliac arteries.The treatment lasted for 28 days in each group.After the treatment,the APO experiment was conducted to measure the testicular and epididymal mass and organ indexes,sperm concentration,and sperm viability in each group of rats.HE staining was performed on the penile corpus cavernosum and testicular tissues of the rats.The levels of NO and cGMP in the penile corpus cavernosum tissue were measured using biochemical assay kits and ELISA.The expression of apoptosis-related proteins in the penile corpus cavernosum tissue was detected using Western blot analysis.Results Compared to the model group,the sildenafil and niang-mu-liang interventions significantly increased the number of erections,as well as the testicular and epididymal mass and indexes,sperm count,and sperm viability in the ED rats.The histological morphology of the penile corpus cavernosum and testicular tissues showed significant improvement.The levels of NO and cGMP in the penile corpus cavernosum tissue were increased.Additionally,the protein expression levels of Bax/Bcl-2,cleaved-caspase3,and cleaved-caspase9 were decreased.Conclusion Niang-mu-liang can improve erectile function in ED rats by inhibiting the apoptotic signaling pathway.