受棉铃虫诱导的棉花糖基转移酶UGT基因家族的生物信息学及表达分析

Bioinformatics and expression analysis of UGT gene family of cotton glycosyltransferase damaged by Helicoverpa armigera

【目的】筛选分析受棉铃虫诱导的棉花糖基转移酶(Glycosyltransferase)UGT基因家族,为UGTG01、UGTG02、UGTG o 4、UGTG o 6、UGTG o 7基因功能深入研究奠定基础。【方法】以受棉铃虫诱导筛选出的棉花糖基转移酶UGT基因组数据库为基础,利用生物信息学方法分析棉花糖基转移酶UGT基因家族的理化性质、系统进化树、蛋白质的二级结构预测以及保守基序与结构域。【结果】从棉花基因组中搜索到5个推定的UTG基因家族成员,其氨基酸数目介于468~534个,理论等电点分布在4.97~5.58,相对分子质量区域为52743.1~60208.62,亚细胞定位在细胞质外,均为亲水性蛋白质。基因结构均含有1个外显子,二级结构主要由α-螺旋、无规则卷曲为主,均含3个Motif基序和有Glycosyltransferase_GTB-type superfamily结构域。5个基因在根、茎、子叶、真叶、花、棉桃中具有明显的组织表达差异性,5个基因与UGT73C家族基因,水稻的UGT707A3、大豆的GmUGT在进化树的同一分支上。【结论】糖基转移酶基因在不同组织中的表达具有显著差异,5个糖基转移酶与UGT73C、UGT707A3、GmUGT都处于进化树的同一个分支上,其可能具有相似的抗虫功能,能通过调控皂苷元、类黄酮、柚皮素等物质响应棉花的抗虫性。

【Objective】This project aims to screen and analyze the UGT gene family of cotton glycosyltransferase damaged by Helicoverpa armigerawere in the hope of providing a basis for in-depth researches into the roles of UGTG01,UGTG02,UGTG o 4,UGTG o 6,and UGTG o 7 genes.【Methods】Based on the genome database of cotton glycosyltransferase UGT damaged by Helicoverpa armigera,the bioinformatics method was used to systematically analyze the physical and chemical properties,phylogenetic tree,secondary structure prediction,conserved motif,and conserved domain of cotton glycosyltransferase UGT gene family.【Results】Five putative members of the UTG gene family with amino acid numbers ranged from 468 to 534,whose theoretical isoelectric point distributions of was from 4.97 to 5.58,relative molecular mass ranged between 52,743.1 and 60,208.62,subcellular localization outside the cytoplasm,and hydrophilicity as proteins were discovered through a genome search of cotton.The gene structure contained one exon,and the secondary structure was mainly composed ofα-helix,irregularly coiled,with three motifs and a Glycosyltransferase_GTB-type superfamily domain.Expression analysis showed that the five genes displayed notable variations in the root,stem,cotyledon,true leaf,flower,and cotton boll;molecular evolutionary tree exhibited that the five UGTs were found to be on the same branch of the UGT73C family genes,rice UGT707A3 and soybean GmUGT.【Conclusion】These glycosyltransferase genes have markedly varied levels of expression in various tissues and are found to be on the same evolutionary branch as UGT73C,UGT707A3,and GmUGT in evolutionary tree analysis,which indicates that they may have similar insect resistance functions,and also respond to insect resistance in cotton by regulating saponin elements,flavonoids,and naringenin.