新疆褐牛PPARγ、MYF5和AGPAT5基因SNP突变与体尺性状的关联分析

A study of association analysis on gene PPARγ,MYF5 and AGPAT5 with body size traits in Xinjiang brown cattle

【目的】鉴定与牛体尺性状相关的基因,筛选PPARγ、MYF5和AGPAT5三个基因的SNP多态性,研究SNP突变对牛体尺性状的影响,为新疆褐牛的分子育种提供标记信息。【方法】采用PCR-RFLP方法对新疆褐牛PPARγ、MYF5和AGPAT5基因的突变位点进行研究,通过单倍型法构建三个目标基因的单倍型块并统计不同单倍型的基因型频率。【结果】三个基因的6个SNP位点对体尺性状均有显著影响,mRNA二级结构预测发现PPARγ-exon7(G74T)和AGPAT5-exon7(G72A)均导致mRNA二级结构和自由能发生改变,蛋白二级结构预测发现牛PPARγ基因外显子1和AGPAT5外显子7的2个SNP位点均为错义突变,改变氨基酸结构,但是蛋白质二级结构未发生改变;MYF5外显子1的SNP位点突变为同义突变,氨基酸及蛋白质二级结构均未发生改变。【结论】筛选PPARγ、MYF5和AGPAT5基因的6个SNP位点,基因PPARγ、MYF5和AGPAT5与牛体尺性状的关系。

【Objective】To screen SNP polymorphisms in PPARγ,MYF5 and AGPAT5 closely related to the regulation of muscle growth and development,lipid metabolism and meat quality,and reveal the effects of SNP mutations on body size traits in Xinjiang brown cattle.【Methods】The mutation of gene PPARγ,MYF5 and AGPAT5 in Xinjiang brown cattle were screened by PCR-RFLP method.The haplotype blocks of the above three target genes were constructed by haplotype method,and the genotype frequencies of different haplotypes were counted.【Results】SNP association analysis showed that six SNP loci of the three genes had structure PPARγ-Exon7(g74t)and AGPAT5-exon7(g72a)led to changes in mRNA secondary structure and free energy,and protein secondary structure prediction revealed that two SNPs in both exon 1 of PPARγand exon 7 of AGPAT5 were missense mutations,which could change amino acid,but no impact on protein secondary structure.【Conclusion】In this study,six polymorphisms in PPARγ,MYF5,and AGPAT5 genes were screened,and through genotyping,association analysis and bioinformatics analysis,and marker AGPAT5,PPARγ,MYF5 and AGPAT5 determined the relationship with body size traits by information for molecular breeding of Xinjiang brown cattle.