响应面法优化柞蚕蛋白粉的制备工艺及有效成分测定

Optimization of preparation process of tussah protein powder by response surface methodology and its effective components determination

以柞蚕粉为原料,脂肪得率和蛋白质得率为评价指标,在单因素试验基础上,响应面法优化柞蚕粉抽提脱脂工艺和脱脂柞蚕粉碱液溶解工艺。结果表明,柞蚕粉抽提脱脂工艺的最佳条件是抽提溶剂为甲醇与正己烷(1∶1,v/v)、料液比1∶14(g/mL)、抽提时间6 h、抽提温度75℃,在此条件下进行验证试验,脂肪得率为20.32%;脱脂柞蚕粉碱液溶解工艺的最佳条件是料液比1∶15(g/mL)、NaOH质量分数0.8%、搅拌时间2 h,在此条件下进行验证试验,蛋白质得率为3.42%。柞蚕蛋白粉中蛋白质含量为80.12%、水分含量为14.44%、灰分含量为2.09%,成品中蛋白质含量高,具有重要的应用价值。

Using tussah powder as raw material,fat yield and protein yield were used as evaluation indexes,on the basis of single factor test,extraction degreasing technology of tussah powder and the alkali solution technology of degreased Tussah powder were optimized by response surface methodology.The results showed that the optimal conditions of extraction degreasing technology of tussah powder were:extraction solvent was methanol and N-hexane(1∶1,v/v),material-liquid ratio 1∶14(g/mL),extraction time 6 h,extraction temperature 75℃,the verification test under these conditions showed that fat yield was 20.32%.The optimal conditions of the alkali solution technology of degreased tussah powder were material-liquid ratio 1∶15(g/mL),NaOH mass fraction 0.8%,stirring time 2 h,the verification test under these conditions showed that protein yield was 3.42%.Protein content was 80.12%,water content was 14.44%,ash content was 2.09% in tussah protein powder and the protein content was high,which had important application value.