TNF-α基因多态性与幽门螺杆菌感染消化不良的关联

Association of TNF-αgene polymorphisms with dyspepsia in Helicobacter pylori infection

目的研究肿瘤坏死因子-α(TNF-α)基因多态性与幽门螺杆菌(Hp)感染消化不良的关联。方法选取2020年3月-2021年3月杭州市富阳区第一人民医院收治的Hp感染消化不良患者110例作为研究组,选取同期于医院健康体检的健康志愿者80名作为对照组;分析TNF-α基因多态性,研究TNF-α基因多态性与Hp感染消化不良的相关性。结果研究组在rsl800629位点GA基因型、rs361525位点GA基因、rsl800630位点CA基因型分布频率低于对照组,rsl800629位点AA基因型、rs361525位点AA基因型、rsl800630位点AA基因型分布频率高于对照组(P<0.05);研究组rsl800629位点G等位基因、rsl800630位点C等位基因携带率低于对照组(P<0.05);多元Logistic回归显示,rsl800629位点A等位基因、rsl800630位点A等位基因与Hp感染消化不良发病有关(P<0.05);转归不佳组在rsl800629位点GA基因型、rsl800630位点CA基因型分布频率低于转归良好组,rsl800629位点AA基因型、rs361525位点AA基因型、rsl800630位点AA基因型分布频率高于转归良好组(P<0.05);转归不佳组rsl800629位点A等位基因、rsl800630位点A等位基因携带率高于转归良好组(P<0.05);多元Logistic回归显示rsl800629位点A等位基因、rsl800630位点AA基因型、A等位基因与患者疾病转归有关(P<0.05)。结论TNF-α基因多态性与Hp感染消化不良有关,rsl800629位点AA基因型、rs361525位点AA基因型、rsl800630位点AA基因型为影响疾病发生和疾病转归的易感基因。

OBJECTIVE To investigate the association of tumor necrosis factor-α(TNF-α)gene polymorphism with dyspepsia of Helicobacter pylori(Hp)infection.METHODS A total of 110Hp patients with dyspepsia admitted to the First People′s Hospital of Fuyang District,Hangzhou from Mar.2020to Mar.2021were selected as the study group,and 80healthy volunteers who took healthy examination in the hospital during the same period were selected as the control group.TNF-αgene polymorphisms were analyzed to investigate the correlation between TNF-αgene polymorphisms and Hp-infected dyspepsia.RESULTS The frequency of distribution of GA genotype at rsl800629,GA gene and CA genotype at rsl800630was lower in the study group than in the control group,and the frequency of distribution of AA genotype at rsl800629,rs361525AA gene and AA gene at rsl800630was higher than in the control group(P<0.05).The carriage rate of G allele at the rsl800629site and C allele at the rsl800630site was lower in the study group than in the control group(P<0.05).Multiple logistic regression showed that the A allele at rsl800629and the A allele at rsl800630were associated with the onset of dyspepsia in Hp infection(P<0.05).The frequency of distribution of GA genotype and CA genotype at rsl800629sites was lower in the poorly regressed group than in the well regressed group,and the frequency of distribution of AA genotype,rs361525,AA and rsl800630sites were higher than in the well regressed group(P<0.05).The carriage rate of allele A and rsl800630site A in rsl800629was higher in the poorly regressed group than in the well regressed group(P<0.05).Multiple Logistic regression showed that the A allele at the rsl800629site,AA genotype at the rsl800630site,and the A allele were associated with disease regression in patients(P<0.05).CONCLUSIONTNF-αgene polymorphisms were associated with dyspepsia in Hp infection,and the AA genotype at rsl800629,AA gene at rs361525,and AA genotype at rsl800630were susceptibility genes affecting disease occurrence and disease regression.