高分辨比色探针快速可视化检测水溶液中铜离子

Rapid visualization and detection of Cu^(2+)in aqueous solution using high-resolution colorimetric probe

目的构建特异性识别水中铜离子(Cu^(2+))并快速显色的化学比色探针。方法基于BODIPY荧光染料设计合成Cu^(2+)比色探针BBSCP,紫外-可见光分光光度法评价探针对水中Cu^(2+)的显色能力,加入常见金属离子后评价比色探针的抗干扰能力。结果BBSCP在485~670 nm波长范围内表现出较宽的吸收带,加入Cu^(2+)后,在465~600 nm区间出现蓝移,波段变尖,而其他金属离子没有引起变化;同时在Cu^(2+)溶液中加入等当量的其他常见金属离子后仍然保持Cu^(2+)显色能力。结论构建的比色探针BBSCP对水溶液中Cu^(2+)具有高度敏感性和选择稳定性,能作为快速检测水中Cu^(2+)的可视化探针广泛应用于生物诊断、食品等多个领域。

Objective To develop a chemical colorimetric probe that specifically recognizes Cu^(2+)in water and then generates color rapidly.Methods Cu^(2+)colorimetric probe BBSCP was designed and synthesized based on BODIPY fluorescence dye,then its color rendering ability to Cu^(2+)in water was evaluated with UV visible spectrophotometry.After common metal ions were added into the Cu^(2+)solution,the anti-interference ability of the colorimetric probe were tested.Results Our developed BBSCP exhibits a wide absorption band in a range of 485 to 670 nm.After the addition of Cu^(2+),a blue shift was observed in the range of 465~600 nm,with sharper band,but no changes were found after other metal ions were added.At the same time,the color rendering ability of Cu^(2+)was maintained even after the addition of equivalent amounts of other common metal ions.Conclusion Our developed colorimetric probe BBSCP has high sensitivity and selective stability to Cu^(2+)in aqueous solution,and can be widely used as a visual probe for rapid detection of Cu^(2+)in water in various fields such as biological diagnosis and food.