反相高效液相色谱法测定大豆磷脂中磷脂酰胆碱含量

Determination of phosphatidylcholine content in soybean phospholipids by reversed phase high performance liquid chromatography method

建立一种通过反相高效液相色谱-紫外测定大豆磷脂中磷脂酰胆碱含量的方法。结果表明:方法的流动相为异丙醇-甲醇-水,且大豆磷脂酰胆碱在0.4615~2.3075 mg/mL范围内线性关系良好(R^(2)=0.9977);方法的检出限为1.4 mg/g、平均回收率为99.8%、相对标准偏差(RSD)为2.41%。方法的精确度高、重复性好,适用于大豆磷脂中磷脂酰胆碱含量的测定。

A method was established for the determination of phosphatidylcholine content in soybean phospholipids by reversed phase high performance liquid chromatography with ultraviolet.The results showed that the mobile phase of the method was isopropanol-methanol-water,the linear relationship of soybean phosphatidylcholine was good in the range of 0.4615-2.3075 mg/mL(R^(2)=0.9977).The detection limit of the method was 1.4 mg/g,the average recovery rate was 99.8%,and the relative standard deviation(RSD)was 2.41%.The method had high accuracy and good repeatability,and was suitable for the determination of phosphatidylcholine content in soybean phospholipids.