顶空气相色谱法分析干姜、细辛及其药对挥发性成分

Volatile Components of Ginger,Asarum and Their Combination by HS-SPME-GC-MS

目的:测定分析比较干姜、细辛及其药对的挥发性成分,为其后续的临床应用提供科学参考。方法:采用顶空固相微萃取-气相色谱-质谱联用(HS-SPME-GC-MS)法,对干姜、细辛及其药对挥发性成分进行提取分析,采用单因素考察法确定最优萃取条件,用面积归一化法计算各成分的相对百分含量,并结合主成分分析(PCA)。结果:从干姜、细辛及药对中共鉴定出78种成分。其中从干姜中鉴定出38种成分,占挥发性总成分的95.13%;从细辛中鉴定出33种成分,占挥发性总成分的97.69%;从药对中鉴定出37种成分,占挥发性总成分的96.31%。3组数据的PCA综合得分差异较大,其中药对得分最高。结论:干姜、细辛及其药对,其挥发性成分及含量均有差异,且两药配伍后产生了新的挥发性成分,因此从挥发性成分变化方面为药对发挥协同增效作用的物质基础研究奠定基础,为其后续药效成分的进一步研究及临床应用提供科学依据。

Objective:To analyze and compare the volatile components of ginger(Rhizoma Zingiberis),asarum(Radixet Rhizoma Asari)and their combination,and provide scientific reference for the follow-up clinical application.Methods:Headspace solid phase microextraction combined with gas chromatography-mass spectrometry(HS-SPME-GC-MS)was employed for extracting and analyzing the volatile components of ginger,asarum and their combination.The optimal extraction conditions were determined by single factor method.The relative percentage of each component was calculated by area normalization method and principal component analysis(PCA)was combined for analysis.Results:A total of 78 volatile components were identified from ginger,asarum and their combination.Among them,38 components were identified from ginger,accounting for 95.13%of the total volatile components;33 were identified from asarum,accounting for 97.69%;37 were identified from the combination,accounting for 96.31%.The PCA comprehensive scores of the three groups were significantly different,and the combination had the highest score.Conclusion:Ginger,asarum,and their combination vary in the types and contents of volatile components,and after the combination of the two herbal medicines produced new volatile components.These findings facilitate the research on the material basis of herbal combination in playing synergistic and effect-enhancing effects,and provide a scientific basis for further research and clinical application of effective components.