石榴皮水不溶性膳食纤维提取及理化性质测定

Extraction and Physicochemical Properties Determination of Water-soluble Dietary Fiber from Pomegranate Peel

以蒙自石榴干皮为原料,采用酸碱结合法提取水不溶性膳食纤维(IDF),在单因素基础上采用正交试验优化提取工艺,并对其理化性质进行测定,结果表明,最佳工艺条件为:碱提取温度55℃、NaOH质量分数6%、碱提液料比19∶1 mL·g^(-1)、碱提取时间50 min;酸提液料比12∶1 mL·g^(-1)、酸提时间60 min、酸提温度60℃。在该工艺条件下IDF提取率为17.81%;持水力和膨胀力分别为4.53 g·g^(-1)、14.10 mL·g^(-1);对饱和脂肪酸和不饱和脂肪酸的吸附量分别为2.73、2.25 g·g^(-1),在模拟小肠的环境下对NO_(2)^(ˉ)及胆固醇吸附能力(175μg·g^(-1),9.21 mg·g^(-1))均比在模拟胃(132μg·g^(-1),3.32 mg·g^(-1))的环境下高。

Using Mengzi the dry peel of pomegranate as raw material,water-soluble dietary fiber(IDF)was extracted using acid-base bonding method.The extraction process was optimized using orthogonal experiments on a single factor basis,and its physicochemical properties were determined.The results showed that the optimal process conditions were:alkali extraction temperature 55℃,NaOH mass frac⁃tion 6%,alkali extraction liquid to material ratio 19∶1 mL·g^(-1),and alkali extraction time 50 minutes;Acid extraction liquid to material ratio of 12∶1 mL·g^(-1),acid extraction time of 60 minutes,and acid ex⁃traction temperature of 60℃.Under this process condition,the IDF extraction rate is 17.81%;the water holding capacity and expansion force are 4.53 g·g^(-1) and 14.10 mL·g^(-1),respectively;the adsorption ca⁃pacities for saturated and unsaturated fatty acids are 2.73 and 22.25 g·g^(-1),respectively.In a simulated small intestine environment,the adsorption capacity for NO_(2)^(ˉ)and cholesterol adsorption capacity(175μg·g^(-1),9.21 mg·g^(-1))were all higher than those in the simulated stomach(132μg·g^(-1),3.32 mg·g^(-1))envi⁃ronment.