保健食品中B族维生素的快速分析方法研究

Study on Rapid Analysis Method of B-Group Vitamins in Health Food

目的:建立同时分析烟酰胺、维生素B1、维生素B6、维生素B2且前处理简单的快速检测方法。方法:样品用0.1%三氟乙酸溶液超声提取,C18色谱柱分离,以0.1%三氟乙酸-乙腈为流动相梯度洗脱,紫外检测器检测。结果:方法对4种B族维生素的分离效果明显,分离度全部超过1.5,标准曲线呈直线回归,相关系数大于0.999 5,检测结果与国家标准方法一致。结论:该方法操作更便捷,分析速度快,灵敏度和准确度高,适用于保健食品中4种B族维生素含量的检测。

Objective:To establish a rapid method for the simultaneous analysis of niacinamide,vitamin B1,vitamin B6 and vitamin B2 with simple pretreatment.Method:The samples were extracted with 0.1%trifluoroacetic acid solution by ultrasonication,separated on a C18 column,eluted with 0.1%trifluoroacetic acid-acetonitrile as the mobile phase gradient,and detected by ultraviolet detector.Result:The method was effective for the separation of the four B vitamins,and the separation degree was more than 1.5 for all of them.The standard curve showed linear regression,and the correlation coefficient was more than 0.9995,and the detection results were in agreement with the national standard method.Conclusion:The method is more convenient to operate,fast to analyze,with high sensitivity and accuracy,and is suitable for the determination of the contents of the 4 B vitamins in health food.