摘要
目的建立沙棘籽油软胶囊中4种生育酚的高效液相色谱(HPLC)含量测定方法。方法采用Agilent Poroshell 120 PFP色谱柱(4.6 mm×250 mm,4μm),流动相为甲醇-水(94∶6),检测波长为294 nm,柱温为26℃,流速为0.9 mL·min^(-1)。结果α-生育酚、β-生育酚、γ-生育酚和δ-生育酚分别在1.83~91.48、1.86~93.09、1.84~91.85、1.77~88.70μg·mL^(-1)范围内呈良好的线性关系,平均回收率在96.32%~98.45%之间,RSD在0.48%~1.43%。结论建立的方法重复性好,专属性强,能够用于测定沙棘籽油软胶囊中4种生育酚的含量。
Objective To establish an HPLC method for determining contents of 4 tocopherols in Seabuckthorn Seed Oil Soft Capsules(SSOSC).Methods HPLC analysis was performed on an Agilent Poroshell 120 PFP column(4.6 mm×250 mm,4μm)with the mobile phase of methanol-water(94∶6).The detection wavelength was 294 nm,the column temperature was 26℃,and the flow rate was 0.9 mL·min^(-1).Resultsα-tocopherol,β-tocopherol,γ-tocopherol andδ-tocopherol showed good linear relationships in the ranges of 1.83~91.48,1.86~93.09,1.84~91.85,1.77~88.70μg·mL^(-1),respectively.The average recoveries was between 96.32%~98.45%with RSD between 0.48%~1.43%.Conclusion The established method has good repeatability and specificity,and could be used to determine contents of 4 tocopherols in SSOSCs.
作者
董蓬
王小兵
李启艳
吴晓云
于海英
谢强胜
郭东晓
DONG Peng;WANG Xiaobing;LI Qiyan;WU Xiaoyun;YU Haiying;XIE Qiangsheng;GUO Dongxiao(NMPA Key Laboratory for Quality Control of Cosmetics Raw Material,Jinan Engineering Research Center for Cosmetics of Specialty Plant Resources,Shandong Institute for Food and Drug Control,Jinan 250101,China)
出处
《药学研究》
CAS
2023年第10期798-800,806,共4页
Journal of Pharmaceutical Research
基金
山东省药品监督管理局检验检测能力提升项目(No.SDNMPAFZLY202205)
国家药品监督管理局化妆品标准研究(No.2021-BZ-014)
国家市场监督管理总局食品审评中心课题(No.ZBW-2018-BJ-03、ZBW-2017-BJSP-11)。
关键词
沙棘
沙棘籽油软胶囊
生育酚
高效液相色谱法
含量测定
Hippophae rhamnoides
Seabuckthorn Seed Oil Soft Capsules
Tocopherol
HPLC
Content determination