沙枣花多糖提取工艺优化及其对益生菌的增殖作用

Optimization of Extraction Process of Elaeagnus angustifolia Flower Polysaccharide and Its Proliferation on Probiotic

为探究沙枣花多糖的最佳提取工艺及其体外对益生菌增殖的影响。实验以沙枣花为原料,利用超声波辅助热水浸提法提取沙枣花多糖,分析料液比、超声时间、提取时间和提取温度对沙枣花多糖得率的影响,运用响应面法对提取条件进行优化,并比较不同多糖质量浓度(0、0.5%、1.0%、1.5%、2.0%和3.0%)对三种益生菌增殖及产酸情况的影响。结果显示,沙枣花多糖最佳提取条件为:料液比1:25 g/mL、超声时间21 min、提取温度72℃、提取时间62 min,得到多糖得率为12.45%±0.15%,与理论预测值(12.587%)相近。当沙枣花多糖质量浓度为2%时,嗜酸乳杆菌、两歧双歧杆菌和青春双歧杆菌的培养基中OD值达到最大,分别为1.23±0.01、1.06±0.02和1.22±0.02,并且pH降到最低(5.17±0.04、5.95±0.04和5.52±0.02),在该浓度下培养40 h后,三种益生菌的生长均趋于稳定。说明沙枣花多糖促进了三种益生菌的增殖和产酸活性。因此,沙枣花多糖可以作为一种潜在的益生元被开发,这为进一步地研究和利用沙枣花资源提供了理论基础。

The study aimed to explore the optimal conditions for the extraction of polysaccharide from Elaeagnus angustifolia flower and its effect on probiotic proliferation in vitro.Polysaccharide was extracted from Elaeagnus angustifolia flower using hot water and ultrasonication.The effects of the material-liquid ratio,duration of ultrasonication,extraction time and extraction temperature on the polysaccharide yield were analyzed.The extraction conditions were optimized by response surface methodology,and the effects of different polysaccharide concentrations(0,0.5%,1.0%,1.5%,2.0%,and 3.0%)on the proliferation and acid production of three probiotics were compared.The results showed that the optimal extraction conditions of Elaeagnus angustifolia flower polysaccharide were as follows:Material-liquid ratio,1:25 g/mL,duration of ultrasonication,21 min,extraction temperature,72℃,extraction time,62 min.The polysaccharide yield was 12.45%±0.15%,which was close to the theoretical predicted yield(12.587%).The highest OD values of Lactobacillus acidophilus,Bifidobacterium bifidum,and Bifidobacterium adolescentis were obtained at a polysaccharide mass concentration of 2%,being 1.23±0.01,1.06±0.02,and 1.22±0.02,respectively,and the lowest pH values(5.17±0.04,5.95±0.04,and 5.52±0.02,respectively).The growth of the three probiotics stabilized after the incubation time reached to 40 h.It indicated that Elaeagnus angustifolia flower polysaccharide promoted the proliferation and acid production of three probiotics.These findings indicate the potential of the polysaccharide from Elaeagnus angustifolia flower as a prebiotic and provide a theoretical basis for further research and the utilization of Elaeagnus angustifolia flower resources.