有机酸种类对罗非鱼皮酸溶性胶原提取和性质的影响

Effects of Organic Acids on the Extraction and Properties of Acid-soluble Collagen from Tilapia Skin

本文比较了醋酸(AA)、酒石酸(TA)、柠檬酸(CA)提取的罗非鱼皮酸溶性胶原(ASC)的结构和性质。利用圆二色光谱(CD)仪和傅里叶变换红外光谱(FTIR)仪分析胶原的二级结构,利用差式扫描量热(DSC)仪测定胶原的热稳定性,并比较分析ASC的成纤维能力。结果表明,AA-ASC、TA-ASC、CA-ASC的提取率分别为15.87%、17.69%和28.63%。CD结果表明AA-ASC和CA-ASC的三螺旋构象含量接近,高于TAASC。FTIR数据表明AA-ASC的氢键含量最多,其次是CA-ASC和TA-ASC。根据DSC结果,发现三种胶原热转变温度(T_(m))没有明显的差异,但AA-ASC、TA-ASC和CA-ASC的焓值分别为1.18、0.73和0.35 J/g。根据成纤维实验结果,发现AA-ASC的成纤维速率和吸光度值最大,其次是CA-ASC和TA-ASC。研究结果表明,有机酸种类对胶原的提取和性质影响可能与羧酸数目和解离常数密切相关。

The effects of three organic acids,namely,acetic acid(AA),tartaric acid(TA),and citric acid(CA),on the structure and properties of acid-soluble collagen(ASC)from tilapia skin were compared in this study.The secondary structure of the collagen samples was analyzed by circular dichroism(CD)spectrometry and Fourier transform infrared spectroscopy(FTIR).The thermal stability of the collagen samples was determined by differential scanning calorimetry(DSC),and the fibroblast ability of ASC was analyzed.Results showed that,the extraction yields of AA-ASC,TA-ASC and CA-ASC were 15.87%,17.69%,and 28.63%,respectively.The content of triple helical conformation of AA-ASC was similar to that of CA-ASC,which was higher than that of TA-ASC.The FTIR data showed that AA-ASC had the highest hydrogen bond content,followed by CA-ASC and TA-ASC.No significant difference was found in the thermal transition temperature(T_(m))of the extracted three collagens,but the enthalpy values of AA-ASC,TA-ASC,and CA-ASC were 1.18,0.73,and 0.35 J/g,respectively.The fibrogenesis experiment revealed that AA-ASC had the highest fibril-forming rate and absorbance,followed by CA-ASC and TA-ASC.The results of this study suggest that the number and dissociation constant of carboxylic acids may play an important role in the effect of organic acids on the extraction and properties of collagen from tilapia skin.