响应面法优化白英粗多糖提取工艺及其体外抗氧化活性的分析

Optimization of Solanum lyratum Crude Polysaccharide Extraction Process Using Response Surface Methodology and Analysis of Its In Vitro Antioxidant Activity

为了优化白英多糖的提取工艺,探究其体外抗氧化活性,本研究采用响应面法对白英多糖的超声提取工艺进行优化:通过单因素实验探究提取温度、提取时间、料液比3个因素对白英多糖得率的影响;在此基础上,采用响应面法优化提取工艺,同时应用红外光谱对白英多糖结构进行分析,并检测其体外抗氧化活性。结果表明,白英多糖的最佳提取工艺为:料液比为1:57 g/mL、提取时间58 min、提取温度65℃,在此条件下白英多糖得率为7.54%±0.12%。红外光谱分析表明白英多糖具有典型的多糖结构。白英多糖对DPPH和ABTS^(+)自由基的半抑制浓度(IC_(50))分别为1.104、1.408 mg/mL,表明其具有良好的体外抗氧化活性。本研究为白英多糖的开发利用提供了理论依据。

To optimize the extraction process of polysaccharide form Solanum lyratum and investigate its in vitro antioxidative activity.In this study,response surface methodology was employed to optimize the ultrasonic-assisted extraction process of polysaccharides from Solanum lyratum.Single-factor experiments were conducted to explore the effects of three factors:Extraction temperature,extraction time,and solid-liquid ratio on the yield.Fourier transform infrared spectroscopy(FTIR)was used to analyze the structure of Solanum lyratum polysaccharide,and in vitro antioxidant activity was investigated.The results showed that the optimal extraction conditions were the material-liquid ratio of 1:57 g/mL,the extraction time of 58 min,and the extraction temperature of 65℃.Under these conditions,the yield of polysaccharides was 7.54%±0.12%.Fourier transform infrared spectroscopy of the extracted polysaccharides exhibited a typical polysaccharide absorbance.Moreover,the half-maximal inhibitory concentrations(IC_(50))of polysaccharides against DPPH and ABTS^(+)free radicals were 1.104 and 1.408 mg/mL,respectively,indicating significant in vitro antioxidant activity.The results of this study provided a theoretical foundation for the further development and utilization of Solanum lyratum polysaccharides.