D-α-生育酚醋酸酯复配物对D-半乳糖致衰老小鼠的改善作用

Ameliorative Effect of D-α-Tocopherol Acetate Complexes on DGalactose-Induced Aging in Mice

为探究D-α-生育酚醋酸酯复配物对D-半乳糖致衰老小鼠的改善作用,通过测定天然油脂复配物+植物甾醇组(VEO组)、D-α-生育酚醋酸酯+植物甾醇复配组(VEZ组)、D-α-生育酚醋酸酯+植物甾醇+虾青素复配组(VEX组)的体外抗氧化能力,并采用小鼠颈背注射D-半乳糖建立衰老模型,同时用不同复配物进行干预。结果表明,三组复配物均有较强的抗氧化作用,其中VEZ组的体外抗氧化效果较佳;与衰老模型小鼠相比,经三组复配物干预后,小鼠体内谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)、总抗氧化能力(Total antioxidant capacity,T-AOC)升高,丙二醛(Malondialdehyde,MDA)生成量下降(P<0.01),血清中炎症因子白介素-1β(Interleukin-1β,IL-1β)、白介素-6(Interleukin-6,IL-6)、肿瘤坏死因子-α(Tumor necrosis factor,TNF-α)和肝功能指标谷丙转氨酶(Alanine aminotransferase,ALT)、谷草转氨酶(Aspartate aminotransferase,AST)水平均显著下降(P<0.01);经过干预后,小鼠体内的核因子-E2-相关因子(Nuclear factor-erythroid 2-related factor 2,Nrf2)、醌氧化还原酶(Quinone oxidoreductase,NQO-1)、血红素氧合酶-1(Heme Oxygenase-1,HO-1)的mRNA和蛋白表达显著增强(P<0.0001),说明不同复配物通过上调Nrf2、NQO-1、HO-1的表达,发挥其抗氧化作用,从而达到抗衰老的效果,其中VEZ组表达效果最佳。综上,D-α-生育酚醋酸酯复配物是通过增加抗氧化相关mRNA和蛋白表达量,从而增强下游抗氧化酶水平来实现抗衰老的效果,其中D-α-生育酚醋酸酯与植物甾醇复配的效果更佳。

To investigate the ameliorative effect of the D-α-tocopheryl acetate compound on D-galactose-induced aging in mice,the in vitro antioxidant capacity of the compound of natural oils+phytosterols(VEO),the compound of D-α-tocopheryl acetate+phytosterol(VEZ),and the compound of D-α-tocopheryl acetat+phytosterol+astaxanthin(VEX)were measured.The aging model was established using mice injected with D-galactose on the back of the neck,while the intervention was carried out with different compounds.The results showed that all three groups of compounds had strong antioxidant effects,with the VEZ group showing better in vitro antioxidant effects.Compared with the aging model mice,the intervention of the three compounds increased glutathione peroxidase(GSH-Px)and total antioxidant capacity(T-AOC),decreased malondialdehyde(MDA)(P<0.01),and a decrease in the serum inflammatory factors interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor(TNF-α)and liver function indicators alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels were significantly reduced(P<0.01).After the intervention,the mRNA and protein expression of nuclear factor-erythroid 2-related factor 2(Nrf2),quinone oxidoreductase(NQO-1)and heme oxygenase-1(HO-1)in mice were significantly enhanced(P<0.0001).This indicated that the different combinations exerted their antioxidant effects through up-regulating the expression of Nrf2,NQO-1 and HO-1,thus achieving anti-aging effects,with the VEZ group showing the best expression effect.In conclusion,D-α-Tocopheryl acetate complex achieved their anti-aging effects by increasing the expression of antioxidant-related mRNAs and proteins,thus enhancing the levels of downstream antioxidant enzymes,among which D-α-tocopheryl acetate was more effective when combined with phytosterols.