沉默维甲酸诱导蛋白14蛋白在胃癌组织芯片中的表达及其临床意义

High expression of retinoic acid induced 14 predicts a poor prognosis for patients with gastric cancer

目的探讨沉默维甲酸诱导蛋白14(RAI14)在胃癌组织的表达及临床意义。方法选择带有随访信息的胃癌组织芯片(HStmA180Su18),采用用免疫组织化学二步法检测癌组织和癌旁组织中RAI14的表达水平,用SPSS 21.0软件分析RAI14表达水平与临床病理特征及癌预后关系进行统计学分析。结果RAI14主要在胞质中表达,癌组织中RAI14表达量显著高于癌旁组织(9.59±2.78比3.03±1.54,P<0.01);RAI14表达水平与病理分化程度(rs=0.280,P<0.01)、肿瘤浸润深度(rs=0.353,P<0.01)、淋巴结转移(rs=0.514,P<0.01)、临床分期(rs=0.496,P<0.01)正相关。Kaplan-Meier生存率分析显示胃癌患者总生存率与RAI14表达负相关(χ^(2)=55.984,df=2,P<0.01)。多因素COX回归分析显示RAI14高表达[比值比(OR)=1.223,95%可信区间(CI):1.124~1.361,P<0.01]和临床分期(OR=3.118,95%CI:1.897~5.539,P<0.01)为影响总生存率的单因素独立危险因素。结论RAI14是胃癌潜在的致癌基因,是胃癌预后的独立风险因素之一。

Objective To elaborate the expression of retinoic acid induced 14(RAI14)in gastric cancer and its clinical implication.Methods We chose a gastric cancer tissue microarray with follow-up information(containing 94 gastric cancer tissues and 86 para-carcinoma tissues).Immunohistochemistry was used to investigate the expression level of RAI14.The relationship between RAI14 expression and clinical index as well as the prognosis of gastric cancer was statistically analyzed by SPSS 21.0 software.Results RAI14 was specifically expressed in the cytoplasm of gastric cancer tissue and para-carcinoma tissue,and there was a significantly positive correlation between them(9.59±2.78 vs.3.03±1.54,P<0.01).The correlation analysis showed that the expression of RAI14 in gastric cancer tissue was significantly positively correlated with pathological grading(rs=0.280,P<0.01),T stage(rs=0.353,P<0.01),N stage(rs=0.514,P<0.01),clinical stages(rs=0.496,P<0.01).Further survival analysis showed that the expression of RAI14 in gastric cancer tissue was significantly negatively correlated with overall survival time of patients,and it was an independent predict factor(χ^(2)=55.984,df=2,P<0.01).In addition,RAI14[odds ratio(OR)=1.223,95%confidence interval(CI):1.124-1.361,P<0.01]and clinical staging(OR=3.118,95%CI:1.897-5.539,P<0.01)were all independent predict factors of gastric cancer patients,and they were significantly negatively correlated with prognosis.Conclusion We hypothesized that RAI14 was a potential oncogene in gastric cancer and it reduced the survival time of patients.