摘要
Base pair mismatch has been regarded as the main source of DNA point mutations, where minor shortlived tautomers were usually involved. However, the detection and characterization of these unnatural species pose challenges to existing techniques. Here, by using systematic structural and ultrafast resonance Raman(RR) spectral analysis for the four possible conformers of guanine-cytosine base pairs, the prominent marker Raman bands were identified. We found that the hydrogen bonding vibrational region from 2300 cm^(-1) to 3700 cm^(-1) is ideal for the identification of these short live species. The marker bands provide direct evidence for the existence of the tautomer species, thus offering an effective strategy to detect the short-lived minor species. Ultrafast resonance Raman spectroscopy would be a powerful tool to provide direct evidence of critical dynamical details of complex systems involving protonation or tautomerization.
基金
the financial support from the National Key Research and Development Program of China(No.2019YFA0708703)
the National Natural Science Foundation of China(NSFC,No.21773309)
the High-level Science Foundation of Qingdao Agricultural University(No.663/1114351)
the Hefei National Laboratory for Physical Sciences at the Microscale(No.KF2020004)
Xiangyang Science and Technology Research and Development(No.2020YL09)
Hubei University of Arts and Science(Nos.HLOM222003,2020kypytd002)。