S100P、GPC3在胃癌中的表达及其与病理特征的关系

Expression of S100P and GPC3 in gastric cancer tissues and its relationship with pathological features

目的研究S100钙结合蛋白(S100P)、磷脂酰肌醇蛋白多糖3(GPC3)在胃癌中的表达水平及其与病理特征的关系。方法选取2020年1月至2022年12月于河南科技大学第一附属医院行手术治疗的95例胃癌患者作为胃癌组,同期选取60例于医院体检的健康人群作为对照组。采用酶联免疫吸附法检测两组受检者的血清S100P、GPC3水平;取胃癌患者术中胃癌组织及癌旁组织,采用试剂盒检测两组织中增殖与侵袭基因[蛋白酪氨酸磷酸酶(PTEN)、存活蛋白(survivin)、细胞周期蛋白依赖性激酶抑制剂1(p21)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)]含量;采用Pearson法分析S100P、GPC3水平与胃癌增殖与侵袭基因表达的相关性,分析S100P、GPC3水平与患者临床病理特征关系,采用Logistic回归模型分析胃癌病理分级的危险因素。结果胃癌组患者的血清S100P水平为(11.87±3.49)μg/L,明显低于对照组的(17.53±4.11)μg/L,GPC3水平为(5.29±1.71)ng/mL,明显高于对照组的(3.31±0.54)ng/mL,差异均有统计学意义(P<0.05);胃癌组织中的Survivin蛋白含量为(77.56±10.35)ng/mg,明显高于癌旁组织的(45.28±7.14)ng/mg,PTEN、p21、Caspase-3蛋白含量分别为(24.17±4.53)ng/mg、(30.48±4.57)ng/mg、(11.36±1.62)ng/mg,明显低于癌旁组织的(38.45±6.19)ng/mg、(47.82±7.20)ng/mg、(18.75±2.46)ng/mg,差异均有统计学意义(P<0.05);Pearson法分析结果显示,Survivin蛋白表达与S100P呈负相关(r=-0.693,P<0.05),与GPC3呈正相关(r=0.686,P<0.05),PTEN、p21、Caspase-3蛋白表达与S100P呈正相关(r=0.704、0.667、0.652,P<0.05),与GPC3呈负相关(r=-0.745、-0.722、-0.693,P<0.05);S100P、GPC3表达水平与患者TNM分期、病情严重程度有关(P<0.05);Logistic回归分析结果显示,S100P、GPC3表达水平为影响胃癌病理分级的危险因素(P<0.05)。结论胃癌患者血清S100P水平下降,GPC3水平上升,两者与病理分级、增殖侵袭基因表达密切相关,可作为评估胃癌早期的有效生物标志物。

Objective To study the expression of S100 calciumbinding protein P(S100P)and glypican-3(GPC3)in gastric cancer tissues and their relationship with pathological features.Methods Ninetyfive patients with gastric cancer who underwent surgical treatment in the First Affiliated Hospital of Henan University of Science and Technology from January 2020 to December 2022 were enrolled(gastric cancer group).Meantime,60 healthy individuals who underwent physical examination during the same period were enrolled as control group.Serum levels of S100P and GPC3 were measured by enzymelinked immunosorbent assay(ELISA).The gastric cancer tissues and adjacent normal tissues were resected during operation,and the contents of proliferation and invasion genes[protein tyrosine phosphatase(PTEN),survivin,cyclindependent kinase inhibitor 1A(p21),cysteine containing aspartate specific protease-3(Caspase-3)]were detected using kits.Pearson method was used to discuss the correlation of S100P and GPC3 levels with the expression of proliferation and invasive genes in gastric cancer tissues.The relationship between S100P and GPC3 levels and clinicopathological characteristics of patients was explored,and Logistic regression model was used to screen the factors affecting the pathological grading of gastric cancer.Results Patients in gastric cancer group had lower serum S100P level and higher GPC3 level than those of control group,(11.87±3.49)μg/L vs(17.53±4.11)μg/L,(5.29±1.71)ng/mL vs(3.31±0.54)ng/mL,all P<0.05.The protein expression level of Survivin was(77.56±10.35)ng/mg in gastric cancer tissue,which was significantly higher than(45.28±7.14)ng/mg in adjacent normal tissue;the expression levels of PTEN,p21,and Caspase-3 protein were(24.17±4.53)ng/mg,(30.48±4.57)ng/mg,and(11.36±1.62)ng/mg in gastric cancer tissue,which were significantly lower than(38.45±6.19)ng/mg,(47.82±7.20)ng/mg,and(18.75±2.46)ng/mg in adjacent normal tissue;the differences were statistically significant(all P<0.05).Pearson analysis showed that the expression of Survivin protein was negatively correlated with S100P(r=-0.693,P<0.05)and positively correlated with GPC3(r=0.686,P<0.05),while the expression of PTEN,p21,and Caspase-3 protein was positively correlated with S100P(r=0.704,0.667,0.652,all P<0.05)and negatively correlated with GPC3(r=-0.745,-0.722,-0.693,all P<0.05).The expression levels of S100P and GPC3 were correlated with TNM stage and severity of gastric cancer(P<0.05).Logistic regression analysis showed that the expression levels of S100P and GPC3 were risk factors for pathological grading of gastric cancer(P<0.05).Conclusion Patients with gastric cancer have decreased serum S100P and increased GPC3 levels,and both of which are closely correlated with pathological grade and the expression of proliferation and invasion genes.The two indexes can be used as effective biomarkers to assess the early stage of gastric cancer.