猪苓糖苷水解酶家族基因及其差异表达

Glycoside hydrolase family genes and their differential expressions in Polyporus umbellatus

猪苓与蜜环菌建立共生关系时,猪苓菌丝会反侵染并消解蜜环菌菌索,以获取营养物质,糖苷水解酶是参与这一过程的主要酶类之一。本研究从猪苓转录数据库中获得糖苷水解酶家族基因42类、309个;其编码的糖苷水解酶蛋白含各类结构域344个,以糖苷水解酶结构域glyco_hydro为主,共计35类173个。对猪苓糖苷水解酶进行的gene ontology(GO)功能注释结果表明,这些蛋白参与的生物学过程以物质代谢及分解过程为主,特别是多糖等大分子物质代谢;181个猪苓糖苷水解酶具有水解酶活性,这些水解酶活性呈多样性分布。与未被蜜环菌侵染的猪苓部位相比,43个糖苷水解酶基因在蜜环菌侵染的菌核部位中差异表达,编码包括glyco_hydro_48、cellulase和polysacc_deac_1等功能结构域,可能发挥水解几丁质、纤维素酶活性及免疫调节等活性,这为猪苓与蜜环菌互作关系的研究提供了候选基因及蛋白。

The mycelium of Polyporus umbellatus can infect and digest the rhizomorph of Armillaria sp.to obtain nutrients during establishing symbiotic relationship between P.umbellatus and Armillaria sp.and glycoside hydrolase is one of the main enzymes involved in this process.In this study,309 glycoside hydrolase family genes of 42 classes were obtained from the transcription database of P.umbellatus sclerotia.They encoded 344 domains,where the glyco_hydro was the major type with a total of 173 in 35 subclasses.The gene ontology(GO)annotation results showed that these proteins mainly involved in the metabolism and decomposition of substances,especially the metabolism of polysaccharides and other macromolecular substances in biological processes.In molecular function subcategories of GO,181 genes displayed hydrolase activities,showing diversity functions.Compared with the sclerotia un-infected by Armillaria sp.,there were 43 differentially expressed genes(DEGs)in infected sclerotia.Function domains like glyco_hydro_48,cellulase and polysacc_deac_1 were encoded by DEGs encoding polysaccase glyco_hydrochitin,cellulase and polysacc_deac_1 etc.All these data provide the candidate genes and proteins for the further study of interactions between P.umbellatus and Armillaria sp.