香菇单核菌丝生长速度差异的相关基因分析

Analyses of genes related to different mycelial growth rate of Lentinula edodes monokaryons

以双核香菇Lentinulaedodes菌株中分离获得2个亲和的单核体Y0040-1和Y0040-3为材料,在不同的培养基上进行培养,进行差异性状评价和表达差异性分析。结果表明在不同的培养基(PDA和2%木屑PDA)上2个单核体菌丝生长速度都存在差异,其中Y0040-1的生长速度显著高于Y0040-3。进一步分析转录组数据发现,在不同培养基上2个单核体比较组(Y0040-3 vs.Y0040-1)有1633个共同的差异基因,这些共同变化的基因可能是导致两者性状差异的主要原因,这些基因中共同上调的有155个,共同下调的有136个。对这些基因进行注释分析,发现共同上调的基因参与代谢过程中的氨基酸代谢和碳水化合物代谢等,分析木质纤维素酶发现,Y0040-1上调基因数量高于Y0040-3,且Y0040-1中的纤维素降解酶和木质素降解酶表达量都明显高于Y0040-3。

Two compatible monokaryons(Y0040-1 and Y0040-3)isolated from dikaryotic Lentinula edodes were used for evaluating growth rate and gene expression differences.The results showed that the mycelial growth rate of the two monokaryons was different on different mediums(PDA and PDA+2%sawdust),and the growth rate of Y0040-1 was higher than that of Y0040-3.Further analysis of transcriptome indicated that a total of 1633 genes detected had common differentially expressed genes,which might be the shared expression pathways leading to the difference between the two monokaryons.Among these genes,155 genes were up-regulated and 136 down-regulated.Annotation and analysis of these genes showed that the co-up-regulated genes were involved in metabolic activities,of which amino acid metabolism and carbohydrate metabolism were the most enriched.Lignocellulase analysis showed that the number of up-regulated genes in Y0040-1 was higher than that in Y0040-3,and the expression levels of cellulose and lignin degrading enzymes in Y0040-1 strain were higher than those in Y0040-3.