过岗龙的化学成分研究

Study on chemical constituents from stems of Entada phaseoloides

目的研究过岗龙(榼藤子Entada phaseoloides干燥藤茎)的化学成分。方法应用正相硅胶、ODS、Sephadex LH-20凝胶柱色谱以及半制备型HPLC等色谱技术进行系统分离纯化,并通过核磁共振波谱、质谱等技术鉴定单体化合物的结构。结果从过岗龙75%乙醇提取物中共分离得到17个化合物,分别鉴定为3-氧-泰国树脂酸(1)、6β-羟基-3-氧代齐墩果-12-烯-28酸(2)、泰国树脂酸(3)、苏门答腊树脂酸(4)、3β,6β-二羟基齐墩果-11,13(18)-二烯-28-酸(5)、(Z)-4-[3′-(β-Dglucopyranosyloxy)butylidene]-3,5,5-trimethyl-2-cyclohexen-l-one(6)、异落新妇苷(7)、黄杞苷(8)、槲皮苷(9)、3-O-α-L鼠李糖-儿茶素(10)、(−)-epicatechin-3-O-p-hydroxybenzoate(11)、lysidicichin(12)、异补骨脂查耳酮(13)、4-羟基-3-甲氧基苯酚-1-O-β-D-(6′-O-没食子酰基)葡萄糖苷(14)、1,6-二-O-没食子酰基-β-D-葡萄吡喃糖(15)、苯甲酸(16)和没食子酸甲酯(17)。结论所有化合物均为首次从过岗龙中分离得到,为中药过岗龙的开发利用提供了一定的物质基础。

Objective To study the chemical constituents from the stems of Entada phaseoloides.Methods The chemical constituents were isolated and purified by silica gel,ODS,Sephadex LH-20 gel column chromatography and semi-preparation HPLC.The structures of all isolates were determined on the basis of NMR and MS analysis.Results A total of 17 compounds were isolated and identified as 3-oxo-siaresinolic acid(1),6β-hydroxy-3-oxo-olean-12-en-28-oic acid(2),siaresinolic acid(3),sumaresinolic acid(4),3β,6β-dihydroxy olean-11,13(18)-dien-28-acid(5),(Z)-4-[3′-(β-D-glucopyranosyloxy)butylidene]-3,5,5-trimethyl-2-cyclohexen-l-one(6),isoastibin(7),engeletin(8),quercitrin(9),catechin-3-O-α-L-rhamnopyranoside(10),(−)-epicatechin-3-O-p-hydroxybenzoate(11),lysidicichin(12),isobavachalcone(13),4-hydroxy-3-methoxy-phenol-1-O-β-D-(6′-O-galoyl)glucopyranoside(14),1,6-di-O-galloyl-β-D-glucopyranoside(15),benzoic acid(16),and gallic acid methlyl ester(17).Conclusion All compounds are isolated from the stems of E.phaseoloides for the first time.This study provides certain study basis for the utilization of E.phaseoloides.