基于药性组合的雌激素样作用中药“性-效-理-病”关系研究

Study on“property-efficiency-pharmacology-disease”relationship of traditional Chinese medicine with estrogen-like effect based on medicinal property combination

目的以雌激素样作用中药为例,探究中药药性、功效、药理作用以及临床应用之间的关联关系。方法以中国知网、PubMed等数据库作为信息来源筛选雌激素样作用中药及复方,通过频数统计分析其药性、功效、来源、主治疾病的分布规律,筛选核心药性组合;从药性组合-功效-作用靶标-功能模块-病理变化-疾病的角度诠释雌激素样作用与中药药性、功效、主治的内在关联。结果共筛选得到82味雌激素样作用中药,主要分布在补虚药与活血化瘀药中,多具有活血、补肾的功效,其核心药性组合为温甘肾、温甘肝、平甘肾、寒苦肝和温辛肝。高频药性组合温甘肾包含了研究频次较高的淫羊藿与人参等中药,其活性成分淫羊藿苷、人参皂苷等可通过调控雌激素受体信号通路、丝裂原活化蛋白激酶(mitogen activated protein kinases,MAPK)级联激活与钙离子的转运从而发挥雌激素样作用。结论核心药性组合温甘肾通过参与细胞自噬与凋亡、腺体发育、免疫调节、糖代谢、骨代谢等生物过程发挥雌激素样作用,防治围绝经期综合征、卵巢早衰、2型糖尿病、肿瘤、神经退行性疾病、骨质疏松等疾病。从分子网络水平阐释了雌激素样作用中药的性-效-理-病关系,为药性理论指导中药临床应用提供科学依据。

Objective Taking traditional Chinese medicine(TCM)with estrogen-like action as an example,the correlation between the medicinal properties,efficiency,pharmacological effects and clinical application of TCM was explored.Methods The TCMs and compounds with estrogen-like effect were screened using CNKI,PubMed and other databases as information sources,the distributions of the properties,effects,sources,and diseases related to the TCM and prescriptions with estrogen-like effect were analyzed by frequency statistics to screen the core property combinations.The internal relationship between the estrogen-like effect and properties,efficacy and indications of TCM was further explained from the perspective of property combinations,efficacy,targets,module of protein interaction networks,pathological processes and diseases.Results A total of 82 TCMs with estrogen-like effect were found,which was mainly distributed among tonifying deficiency,promoting blood circulation,and removing blood stasis drugs,which have the effects of invigorating circulation and reinforcing kidney.Their core property combinations were warm-sweet-kidney,warm-sweetliver,neutral-sweet-kidney,cold-bitter-liver,and warm-pungent-liver.The high-frequency property combination warm-sweet-kidney contained TCMs such as Yinyanghuo(Epimedii Folium)and Renshen(Ginseng Radix et Rhizoma),which have been studied frequently.Their active components such as icariin and ginsenoside could exert estrogen-like effect by regulating estrogen receptor signaling pathways,mitogen activated protein kinases(MAPK)cascade activation and calcium ion transport.Conclusion By participating in the biological processes of autophagy and apoptosis,glandular development,immune regulation,glucose metabolism,bone metabolism,etc.,core property combination warm-sweet-kidney played the estrogen-like role in the prevention and treatment of perimenopausal syndrome,premature ovarian failure,type 2 diabetes,tumor,neurodegenerative diseases,osteoporosis and other diseases.This study explained the relationship among property,effects,pharmacology and diseases of TCM with estrogen-like effect from a molecular network level,providing a scientific basis for guiding its clinical application.