基于多元统计方法和成分差异分析栀子及其炮制品质量

Quality of Gardenia jasminoides and its processed products based on multivariate statistics and component differential analysis

目的 建立栀子Gardenia jasminoides的UPLC指纹图谱,比较栀子及其炮制品之间的差异。方法 收集2个产地10批栀子,使用不同方法炮制,采用Waters Acquity UPLC BEH C18色谱柱(100 mm×2.1 mm,1.7μm),栀子及其炮制品用甲醇超声提取制备溶液,以乙腈-0.2%磷酸水溶液为流动相,梯度洗脱,进样量1μL,柱温30℃,体积流量0.2 mL/min,检测波长为239 nm与440 nm,建立其UPLC指纹图谱。运用总量统计矩相似度、灰色关联度、TOPSIS和主成分分析(principal component analysis,PCA)对栀子的UPLC特征指纹图谱进行数据分析。结果 栀子及其炮制品的UPLC特征指纹图谱的共有标志峰20个,其中4个鉴定为栀子苷、京尼平龙胆双糖苷、西红花苷Ⅰ、西红花苷Ⅱ。2产地的栀子经炮制成焦栀子后,栀子苷、西红花苷Ⅰ与西红花苷Ⅱ含量有所减少。湖南湘乡地区栀子经炮制后其京尼平龙单双糖苷含量有一定增加。结论 所建立的栀子不同炮制品特征指纹图谱共有20个标志峰,不同炮制品之间标志峰峰面积和含量存在差异,为栀子的不同炮制品的质量控制与评价提供了依据。

Objective To establish the UPLC fingerprint of Gardenia jasminoides,so as to compare the difference between G.jasminoides and its processed products.Methods Ten batches of G.jasminoides from two different regions were collected and processed by different methods.The separation was operated on Waters Acquity UPLC BEH C18 chromatographic column(100 mm×2.1 mm,1.7μm).G.jasminoides and its processed products were prepared by ultrasonic extraction with methanol.Acetonitrile and 0.2%phosphoric acid water were used as mobile phases for gradient elution,with injection volume of 1μL,column temperature of 30℃,flow rate of 0.2 mL/min,detection wavelength of 239 nm and 440 nm.The UPLC fingerprint library was established.The UPLC fingerprints of G.jasminoides were analyzed by using total statistical moment similarity,grey correlation,TOPSIS and principal component analysis(PCA).Results There were 20 characteristic peaks in the UPLC fingerprint of G.jasminoides and its processed products,of which four were identified as geniposide,genipin gentianoside,crocin Ⅰ and crocin Ⅱ.The content of geniposide,crocin Ⅰ and crocin Ⅱ was reduced in G.jasminoides processed products(Jiaozhizi,Gardeniae Fructus Praeparatus)from two regions.The content of genipin gentianoside was increased in a certain in G.jasminoides processed products from Xiangxiang in Hunan.Conclusion Twenty characteristic UPLC fingerprints of different processed products of G.jasminoides were established.The fingerprints of different processed products of G.jasminoides have certain differences in peak area and content,.which provide theoretical and experimental basis for the quality control and evaluation of different processed products of G.jasminoides.