药用植物紫红獐牙菜的转录组信息分析

Transcriptome Information Analysis for Medicinal Plant Swertia punicea

紫红獐牙菜是重要的民族药,本研究采用高通量测序技术对紫红獐牙菜进行转录组测序并分析。结果显示,完成的15个样品的转录组测序,获得112.17 Gb Clean data,各样品Clean data均达到6.18 Gb,Q30碱基百分比在94.96%及以上。组装后共获得47 106条unigene,其中长度在1 kb以上的unigene有18 971条。通过与KEGG、GO、KOG、COG等多个数据库进行比对,对unigene进行功能注释,共获得35 375条unigene。GO数据库中注释到的26 570条unigene,可分为生物学过程、细胞组分和分子功能三大类共44个亚类。以KEGG数据库为参考,21 321条基因被注释,参与的代谢通路分为5大类,分别是细胞过程、环境信息处理、遗传信息处理、代谢和有机系统,其中与代谢相关的通路最多,约占所用通路的72.5%;KEGG代谢通路分析发现紫红獐牙菜中1 147条unigene参与到22个次生代谢标准通路中,有77条unigene参与编码环烯醚萜苷类合成通路中的25个关键酶。此外,紫红獐牙菜转录组中找到9 027个SSR重复位点,6种SSR重复类型,最多的为双碱基重复占66%,最少的为六碱基重复,占0.1%。本研究结果填补了紫红獐牙菜的转录组数据空白,为该植物合理利用与品质调控提供一定的帮助。

Swertia purpurea is an important ethnic medicine.The transcriptome of S.punicea was sequenced and analyzed by High-throughput sequencing technology.After completing the transcriptome sequencing of 15 samples,112.17 Gb clean data were obtained.The clean data of all samples reached 6.18 Gb,and the percentage of Q30 base was 94.96%.After assembly,a total of 47106 unigenes were obtained,among which 18971 unigenes were more than 1 kb in length.Compared with KEGG,GO,KOG,COG and other databases,unigene was annotated,and 35375 unigene annotation results were obtained.The 26570 unigenes annotated in GO database can be divided into three categories:biological processes,cellular components and molecular functions,with a total of 44 subclasses.Based on KEGG database,21321 genes were annotated,and the metabolic pathways involved were divided into five categories,including cellular process,environmental information processing,genetic information processing,metabolism and organic system.Among them,the metabolic pathway was the most,accounting for 72.5%of the pathways;KEGG pathway analysis showed that 1147 unigenes were involved in 22 secondary metabolic standard pathways,and 77 unigenes were involved in 25 key enzymes in iridoid glycoside biosynthesis pathway.In addition,we found 9027 SSR repeat sites and 6 SSR repeat types from transcriptome in S.punicea,with the most double base repeat SSR accounting for 66%and the least six base repeat SSR accounting for 0.1%.The results filled in the blank of transcriptional group information of S.punicea,which provide some assistance for the rational utilization and quality control of the plant.