血清FOXP1和CDK2与围绝经期妇女发生骨质疏松的相关性分析

Correlation analysis between serum FOXP1 and CDK2 levels and the occurrence of osteoporosis in perimenopausal women

目的探讨围绝经期骨质疏松(osteoporosis,OP)患者血清叉头框蛋白P1(forkhead box protein P1,FOXP1)和细胞周期蛋白依赖激酶2(cyclin-dependent kinase2,CDK2)与骨密度相关性。方法选取2021年2月至2022年8月于湖州市第一人民医院内分泌科就诊的80例围绝经期妇女作为研究对象,根据是否有骨质疏松分为OP组38例,对照组42例,利用酶联免疫吸附法检测样本血清中FOXP1及CDK2表达含量,DEXA骨密度分析仪检测两组妇女腰椎(L1~L4)及股骨颈骨密度表达水平。采用Pearson法分析围绝经期妇女OP患者血清FOXP1和CDK2与骨密度相关性。结果对照组血清中FOXP1及CDK2含量分别为(42.94±3.37)pg/ml和(102.46±12.25)pg/ml,OP组血清FOXP1及CDK2含量分别为(25.91±4.38)pg/ml和(60.59±10.78)pg/ml;与对照组相比,OP组血清FOXP1和CDK2表达水平显著降低,差异均有统计学意义(P<0.05)。对照组腰椎(L1~L4)和股骨颈骨密度分别为0.68±0.28和0.43±0.99,OP组腰椎(L1~L4)和股骨颈骨密度分别为0.40±0.10和0.21±0.15,与对照组相比,OP组腰椎(L1~L4)和股骨颈骨密度降低,差异均有统计学意义(P<0.05)。相关性分析结果显示,血清FOXP1表达水平与腰椎(L1~L4)及股骨颈骨密度呈正相关(r=0.55、0.25,P<0.05),CDK2表达水平与腰椎(L1~L4)骨密度呈正相关(r=0.60,P<0.05)。结论围绝经期OP患者中,血清中的FOXP1和CDK2表达明显降低,且与骨密度存在相关性,提示FOXP1和CDK2可能可作为评估围绝经期妇女OP病情的生物学指标和临床预测指标。

Objective To investigate the correlation between serum forkhead box protein P1(FOXP1)and cyclin-dependent kinase2(CDK2)and bone mineral density in perimenopausal women with osteoporosis(OP).Methods A total of 80 perimenopausal women treated in our hospital from Feb.2021 to Aug.2022 were selected as study objects.Among them,38 were in the OP group and 42 were in the control group.The expression levels of FOXP1 and CDK2 in serum were detected by ELISA.BMD expression levels of lumbar spine(L1-L4)and femoral neck were measured by DEXA BMD analyzer.The correlation between serum FOXP1 and CDK2 and bone mineral density in perimenopausal women with osteoporosis was analyzed by Pearson method.Results Compared with the control group,the contents of FOXP1 and CDK2 in serum of the control group were(42.94±3.37)pg/ml and(102.46±12.247)pg/ml,respectively.Serum FOXP1 and CDK2 contents in OP group were(25.91±4.38)pg/ml and(60.59±10.777)pg/ml,respectively.Compared with the control group,serum FOXP1 and CDK2 expression levels and bone mineral density of lumbar spine(L1-L4)and femoral neck in OP group were significantly decreased.The BMD of lumbar spine(L1-L4)and femoral neck of women in the control group were 0.68±0.28 and 0.43±0.99,respectively.BMD of lumbar spine(L1-L4)and femoral neck of women in the OP group were 0.40±0.10 and 0.21±0.15,respectively.Bone mineral density decreased in the lumbar spine(L1-L4)and femoral neck in the OP group.The differences were statistically significant(P<0.05).In addition,the correlation analysis results showed that the expression level of FOXP1 in serum was positively correlated with bone mineral density of lumbar spine(L1-L4)and femoral neck(r=0.546,0.245,P<0.05),and the expression level of CDK2 was positively correlated with bone mineral density of lumbar spine(L1-L4)(r=0.600,P<0.05).Conclusions In perimenopausal women with osteoporosis,the expression of FOXP1 and CDK2 in serum was significantly decreased,and there was a correlation with bone mineral density.It is suggested that FOXP1 and CDK2 may be used as biological indicators and clinical predictors of osteoporosis in perimenopausal women.