长链非编码RNA ABHD11-AS1通过调节miR-542-3p/MAP3K11对胃癌细胞侵袭和迁移的影响

Influences of long non-coding RNA ABHD11-AS1 on the invasion and migration of gastric cancer cells by regulating miR-542-3p/MAP3K11

目的探讨长链非编码RNA ABHD11-AS1(long non-coding RNA ABHD11-antisense RNA1,LncRNA ABHD11-AS1)通过调节miR-542-3p/MAP3K11对胃癌(gastric cancer,GC)细胞侵袭和迁移的影响。方法胃癌细胞BGC-823常规培养后依次分为si-NC组、si-LncRNA ABHD11-AS1组、si-LncRNA ABHD11-AS1+miR-NC inhibitor组、si-LncRNA ABHD11-AS1+miR-542-3p inhibitor组、si-LncRNA ABHD11-AS1+miR-542-3p inhibitor+si-con组和ABHD11-AS1+miR-542-3p inhibitor+si-MAP3K11组。预测并验证LncRNA ABHD11-AS1/miR-542-3p/MAP3K11之间的相互作用关系。qRT-PCR检测胃癌细胞中LncRNA ABHD11-AS1、miR-542-3p、MAP3K11 mRNA水平,Transwell法检测胃癌细胞迁移和侵袭能力,Western blot检测胃癌细胞中MAP3K11、MMP-2和MMP-9蛋白表达水平。结果LncRNA ABHD11-AS1和miR-542-3p、miR-542-3p和MAP3K11靶向关系被验证。与si-NC组[(184.09±17.32)和(87.64±7.54)]比较,si-LncRNA ABHD11-AS1胃癌细胞的迁移和侵袭能力明显降低[(68.35±6.21)和(28.23±3.05)(P均<0.05)];与si-LncRNA ABHD11-AS1+miR-NC inhibitor组[(75.39±7.08)和(31.26±3.15)]比较,si-LncRNA ABHD11-AS1+miR-542-3p inhibitor组胃癌细胞迁移和侵袭能力升高[(138.24±12.58)和(61.23±5.86)(P均<0.05)];与si-LncRNA ABHD11-AS1+miR-542-3p inhibitor+si-con组[(145.33±13.09)和(65.45±6.08)]比较,si-LncRNA ABHD11-AS1+miR-542-3p inhibitor+si-MAP3K11组胃癌细胞的迁移和侵袭能力明显降低[(108.36±9.87)和(145.33±13.09)(P均<0.05)]。结论下调LncRNA ABHD11-AS1调控miR-542-3p/MAP3K11轴可抑制胃癌细胞迁移和侵袭。

Objective To investigate the effect of long non-coding RNA ABHD11-antisense RNA1(LncRNA ABHD11-AS1)on the invasion and migration of gastric cancer(GC)cells by regulating miR-542-3p/MAP3K11.Methods After routine culture,gastric cancer cells BGC-823 were divided into si-NC group,si-LncRNA ABHD11-AS1 group,si-LncRNA ABHD11-AS1+miR-NC inhibitor group,si-LncRNA ABHD11-AS1+miR-542-3p inhibitor,si-LncRNA ABHD11-AS1+miR-542-3p inhibitor+si-con group and ABHD11-AS1+miR-542-3p inhibitor+si-MAP3K11 group.The interaction relationship between LncRNA ABHD11-AS1,miR-542-3p,and MAP3K11 was predicted and verified.The levels of LncRNA ABHD11-AS1,miR-542-3p,and MAP3K11 mRNA in GC cells were detected by qRT-PCR,and the migration and invasion ability of GC cells were detected by the Transwell method,and the protein expression levels of MAP3K11,MMP-2,and MMP-9 in GC cells were detected by Western blot.Results The targeting relationship between LncRNA ABHD11-AS1 and miR-542-3p,miR-542-3p and MAP3K11 were verified(P<0.05).Compared with the si-NC group[(184.09±17.32)and(87.64±7.54)],the migration and invasion capacity of gastric cancer cells in the si-LncRNA ABHD11-AS1 group were significantly reduced[(68.35±6.21)and(28.23±3.05)(P all<0.05)];Compared with the si-LncRNA ABHD11-AS1+miR-NC inhibitor group[(75.39±7.08)and(31.26±3.15)],the migration and invasion capacity of gastric cancer cells in the si-LncRNA ABHD11-AS1+miR-542-3p inhibitor group were significantly increased[(138.24±12.58)and(61.23±5.86)(P all<0.05)];Compared with the si-LncRNA ABHD11-AS1+miR-542-3p inhibitor+si-con group[(145.33±13.09)and(65.45±6.08)],the migration and invasion ability of gastric cancer cells in the si-LncRNA ABHD11-AS1+miR-542-3p inhibitor+si-MAP3K11 group were significantly reduced[(108.36±9.87)and(145.33±13.09)(P all<0.05)].Conclusion Down-regulating of LncRNA ABHD11-AS1 could suppress the migration and invasion of GC cells by miR-542-3p/MAP3K11 axis.