乙二醇壳聚糖自愈合水凝胶的制备及性能

Preparation and properties of glycol chitosan based self-healing hydrogel

合成一种基于动态亚胺键的自愈合氧化海藻酸钠-乙二醇壳聚糖水凝胶(OSA-GC),将海藻酸钠通过高碘酸钠氧化合成氧化海藻酸钠(OSA),并与乙二醇壳聚糖(GC)发生席夫碱反应制备具有不同交联度的自愈合OSA-GC水凝胶,研究GC的浓度对OSA-GC水凝胶的微观形态、黏弹性能、溶胀性能、自愈合性能、降解速率和体外药物释放性能的影响。结果表明:OSA-GC水凝胶具有多孔结构,通过控制OSA与GC的质量比,OSA-GC水凝胶的孔径处于50~280μm;OSA-GC水凝胶的溶胀率在120 h达到溶胀平衡,溶胀率达到71.3~112.1。OSA-GC水凝胶在含有溶菌酶(10 mg/mL)的PBS中发生降解,在12天后OSA-GC水凝胶的质量损失达到43.1%~51.9%;在室温条件下,OSA-GC水凝胶在无外界刺激时2 h实现自愈合,负载吉西他滨的OSA-GC自愈合水凝胶对抗癌药物吉西他滨具有缓释作用,药物释放时间可达48 h,药物释放量达到72.6%~89.5%。OSA-GC自愈合水凝胶在药物载体领域具有较好的应用前景。

The self-healing oxidized sodium alginate-glycol chitosan hydrogel(OSA-GC)based on dynamic imine bonds was synthesized.Oxidized sodium alginate(OSA)was synthesized by oxidizing sodium alginate with sodium periodate,and self-healing OSA-GC hydrogels with different cross-linking degrees were prepared by Schiff base reaction with glycol chitosan(GC).The effect of GC concentration on the microscopic morphology,viscoelasticity,swelling performance,self-repair performance,degradation rate and in vitro drug release performance of OSA-GC hydrogels were investigated.The results show that OSA-GC hydrogels have the characteristics of porous structure with a pore size ranging from 50μm to 280μm by controlling the mass ratio of OSA to GC.The OSA-GC hydrogels can reach the swelling equilibrium at 120 hours,and the swelling ratio reach 71.3-112.1.OSA-GC hydrogel can be degraded in PBS containing lysozyme(10 mg/mL),and the mass loss of OSA-GC hydrogel is 43.1%-51.9% after 12 days.At room temperature,OSA-GC hydrogels can achieve self-healing within 2 h in the absence of external stimuli.OSA-GC hydrogel loaded with gemcitabine shows a sustained release effect on the anticancer drug gemcitabine,and the release time can reach 48 hours in the drug release experiment,which shows promising application prospects in biomedical fields of drug carriers.