水杨酸浸种对铝胁迫下多年生黑麦草种子萌发的影响

Effects of salicylic acid seed soaking on seed germi⁃nation of Lolium perenne under different aluminum stress

【目的】探讨水杨酸浸种对不同浓度铝胁迫处理下多年生黑麦草种子萌发的影响。【方法】以多年生黑麦草(Lolium perenne)品种冬牧70(Secale Cerale)种子为试验材料,并将材料分为2组,一组为无水杨酸浸种组,另一组用0.5 mmol/L水杨酸(salicylic acid,SA)浸种。用7个AlCl_(3)浓度(0、0.2、0.5、1.0、2.0、5.0、10.0 mmol/L)处理液对2组黑麦草种子进行处理。【结果】1)无水杨酸浸种组种子发芽势、发芽率并未受到各浓度铝胁迫显著影响。当AlCl_(3)处理浓度达到10.0 mmol/L时,0.5 mmo/L水杨酸浸种组种子发芽势及发芽率显著被抑制。2)2组种子的芽长和根长均随着AlCl_(3)处理浓度的升高而显著降低;3)随着铝胁迫浓度的增加,无水杨酸浸种组多年生黑麦草种子SOD、CAT、POD活性均受到抑制;0.5 mmo/L水杨酸浸种多年生黑麦草种子在铝胁迫下的POD活性增强,但SOD和CAT活性未增强。【结论】水杨酸能够增强铝胁迫下多年生黑麦草抗氧化系统POD活性,但对促进种子萌发不明显。

【Objective】The study is carried out to investigate the effects of salicylic acid seed soaking on seed germination of Lolium perenne under different aluminum stress.【Method】The seeds of perennial ryegrass(Lolium perenne)′Secale Cerale 70′were used as experimental materials and were divided into two groups.One group was soaked without salicylic acid,and the other group was soaked with 0.5 mmol/L salicylic acid(SA).Seven AlCl3 solu⁃tions with different concentrations(0,0.2,0.5,1.0,2.0,5.0,10.0 mmol/L)were used to treat the seeds of the two groups of ryegrass.【Result】The results showed as follows:1)Germination potential and germination rate of seeds in salicylic acid-free soaking group were not significantly affected by various concentrations of aluminum stress.When AlCl3 concentration reached 10.0 mmol/L,seed germination and germination rate were significantly inhibited in 0.5 mmol/L salicylic acid soaking group.2)The seed bud length and root length of both groups decreased significantly with the increase of AlCl3 treatment concentration;3)With the increase of Al stress concentration,the activities of SOD,CAT and POD of perennial ryegrass seeds in salicylic acid free soaking group were inhibited.0.5 mmol/L sali⁃cylic acid soaking increased POD activity of perennial ryegrass seeds under Al stress,but did not increase SOD and CAT activities.【Conclusion】Salicylic acid seed soaking could enhance POD activity of the antioxidant system of fe⁃rennial wild ryegrass under aluminum stress,but the promotion effect on seed gerination was not significant.