基于高效液相色谱法特征图谱和一测多评的蟾酥质量评价

Quality Evaluation of Bufonis Venenum Based on HPLC Specific Chromatograms Combined with QAMS

为了对不同产地蟾酥质量进行对比研究.利用24批蟾酥建立了HPLC特征图谱,比较不同产地蟾酥特征图谱相似度,进行聚类分析和主成分分析.针对特征图谱中的日蟾毒它灵、蟾毒它灵、蟾毒灵、华蟾酥毒基、脂蟾毒配基5个共有峰建立HPLC一测多评方法,测定24批蟾酥中5个成分的含量.结果发现:建立蟾酥特征图谱,确定9个共有峰,指认其中5个共有峰.指纹图谱相似度范围是0.136~0.996,四川样本与其他各省样品相似度最低.相似度分析、聚类分析和主成分分析结果表明,蟾酥样品化学成分具有一定的地域规律性.不同省份蟾酥中日蟾毒它灵、蟾毒它灵、蟾毒灵、华蟾酥毒基、脂蟾毒配基含量相差较大,东北三省及河北、山东、江苏3个东部省份蟾酥样品蟾毒内酯类含量较高,河南、四川省蟾毒内酯类含量较低.不同批次蟾酥5种蟾毒内酯类化合物含量比例差别较大,未体现出明显的地域特性.研究建立了蟾酥特征图谱检测方法及含量一测多评检测方法,为蟾酥药材质量评价研究提供依据.蟾酥中蟾毒内酯类成分的积累具有一定的地域性,对于蟾蜍的养殖及蟾酥药材资源的合理开发应用具有重要指导意义.

To compare and evaluate the quality of Bufonis Venenum from different origins.The high performance liquid chromatography(HPLC)specific chromatograms were established using 24 batches of Bufonis Venenum.The similarity evaluation,cluster analysis(HCA),principal component analysis(PCA)were carried out.A quantitative analysis of multi-components by single marker(QAMS)was established to determine the content of main chemical components in Bufonis Venenum.The specific chromatograms of Bufonis Venenum were established,a total of 9 common peaks were calibrated and 5 of them were identified.The specific chromatograms similarities ranged from 0.136 to 0.996.The samples from Sichuan had the lowest similarity with those from other provinces.The results f similarity evaluation,HCA and PCA showed that the quality of Bufonis Venenum was mainly affected by the factor of geographic location.The contents of gamabufalin,bufotalin,bufalin,cinobufagin,resibufogenin in Bufonis Venenum from different geographical origins were quite different,higher in the samples from Heilongjiang,Jilin,Liaoning,Hebei,Shandong,Jiansu,lower in those from Henan,Sichuan.The proportions of these five bufadienolides compounds in different batches of Bufonis Venenum were also quite different,with no regularity in their origins.The HPLC specific chromatogram method and multi-evaluation method established provide the basis for the research on the quality of Bufonis Venenum.The research showed that the accumulation of bufadienolides had certain regional regularity which is of great significance for the breeding of toads and the rational development of the medicinal resources of Bufonis Venenum.