基于气味分析系统的不同产地秦艽的区分及相关性研究

Identification of Gentianae Macrophyllae Radix from Different Habitats Based on Odor Analysis System

目的:基于电子鼻PEN3系统结合多元统计分析对不同产地秦艽进行鉴别。方法:先通过电子鼻系统动态采集秦艽芳香成分并得到响应值,通过单因素试验确定最佳试验条件,再应用主成分分析(PCA)、线性判别分析(LDA)及负荷加载分析法(Loadings)对结果进行分析。结果:秦艽电子鼻最佳试验条件为样品称样量3.0 g、进气量250 mL·min^(-1)、样品室温放置时间15 min、颗粒度60目。PCA和LDA均对不同产地的秦艽有一定的区分能力,并且LDA优于PCA。结论:电子鼻对秦艽的种类和产地鉴别准确性好且简便可行,能更准确、客观地对不同种、不同产地、不同栽培方式的秦艽进行鉴别。

Objective:To identify the Gentianae Macrophyllae Radix samples from different habitats by using the electronic nose PEN3 system combined with multivariate statistical analysis.Methods:Firstly,the electronic nose system was used to collect the aromatic components of Gentianae Macrophyllae Radix and obtain the response values,and the single-factor test was employed to determine the best experimental conditions.Then,the principal component analysis(PCA),linear discriminant analysis(LDA),and loading analysis were conducted for the obtained data.Results:The best experimental conditions of the electronic nose were as follows:sample weight of 3.0 g,intake flow rate of 250 mL·min^(-1),storage time of 15 mins at room temperature,and the granularity of 60 meshes.PCA and LDA can distinguish the Gentianae Macrophyllae Radix samples from different habitats,and LDA outperformed PCA.Conclusion:The electronic nose had high accuracy of identifying the plant species,habitats,and cultivation methods of Gentianae Macrophyllae Radix,being simple and feasible.