CXCR3基因多态性在重庆两江新区老年人群中慢性肺阻塞性肺疾病易感性的流行病学分析

Epidemiological analysis on the susceptibility of CXCR3 gene polymorphism to COPD in the elderly population in Liangjiang New Area of Chongqing

目的探究和分析CXC趋化因子受体(CXC chemokine receptor 3,CXCR3)基因多态性在重庆两江新区老年人群中慢性肺阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)易感性的流行病学。方法筛选2020年1月至2022年9月重庆两江新区人民医院就诊及治疗的COPD患者,将符合标准的276例患者纳入研究,列为观察组。纳入同时期肺功能健康受诊者512例,列为对照组。分析两组病例资料,并通过SBaPshot技术检测基因分型,分析基因多态性与COPD易感性、临床特征的关系。结果两组患者年龄、性别、BMI和血嗜酸性颗粒细胞水平差异无统计学意义,具有可比性(P>0.05)。吸烟史、肺功能指标、MMP-9和TIMP-1水平差异有统计学意义(P<0.05)。与对照组比较,观察组rs2280964位点的纯合子TT相较于CC发生COPD的风险更高(P<0.05),而在rs34334103位点中两组基因分布差异无统计学意义(P>0.05)。观察组rs2280964位点三种基因分布MMP-9水平差异明显(P=0.003),TIMP-1水平差异无统计学意义(P=0.187);rs34334103位点三种基因分布MMP-9和TIMP-1水平差异无统计学意义(均P>0.05)。rs2280964位点纯合子TT型患者MMP-9水平较纯合子CC型明显增加(P=0.024)。CXCR3 rs34334103位点三种基因分布患者的FEV1/FVC差异有统计学意义(P=0.008),其中CC+CT、TT的隐性模型差异有统计学意义(P<0.01),TT型FEV1/FVC水平最低。其他SNP位点与COPD临床症状无明显相关性(P均>0.05)。结论CXCR3基因多态性与COPD易感性明显相关,同时也与患者血清MMP-9、FEV1/FVC水平明显相关,可作为临床研究和治疗的新靶点。

Objective To explore and analyze the epidemiology of susceptibility to chronic obstructive pulmonary disease(COPD)among the elderly population in Liangjiang New Area of Chongqing based on CXC chemokine receptor 3(CXCR3)gene polymorphism.Methods From January 2020 to September 2022,the Medical Laboratory Department of Chongqing Liangjiang New Area People's Hospital selected COPD patients and received treatment.Among the 276 patients who met the criteria were included in the study and included in the observation group.Among the 512 patients with healthy pulmonary function in the same period were included in the control group.The data of the two groups of patients were analyzed,and the genotypes were detected by SBaPhotoshot technology to analyze the relationship between gene polymorphism and the susceptibility and clinical characteristics of COPD.Results There was no significant difference between the two groups in age,sex,BMI and blood eosinophil granulocyte levels,which was comparable(P>0.05).There were significant differences in smoking history,pulmonary function index,MMP-9 and TIMP-1 levels(P<0.05).Compared with the control group,the homozygous TT of rs2280964 locus in the observation group had a higher risk of COPD than that of CC(P<0.05),but there was no significant difference in gene distribution between the two groups at rs34334103 locus(P>0.05).In the observation group,the MMP-9 level of rs2280964 locus was significantly different(P=0.003),while the TIMP-1 level was not significantly different(P=0.187);There was no significant difference in MMP-9 and TIMP-1 levels among the three genes at rs34334103 locus(all P>0.05).The level of MMP-9 in homozygous TT patients with rs2280964 locus was significantly higher than that in homozygous CC patients(P=0.024).There were differences in FEV1/FVC of patients with CXCR3 rs34,334,103 gene distribution(P=0.008),among which there were significant differences in CC+CT and TT recessive models(P<0.01),and the level of FEV1/FVC of TT type was the lowest.There was no significant correlation between other SNP loci and clinical symptoms of COPD(all P>0.05).Conclusion CXCR3 gene polymorphism is significantly associated with the susceptibility to COPD,and also with the serum levels of MMP-9 and FEV1/FVC,which can be used as a new target for clinical research and treatment.