急性一氧化碳中毒脑损伤患者血清细胞因子的表达特征研究

Expression characteristics of serum cytokines in patients with brain injury after acute carbon monoxide poisoning

目的探讨急性一氧化碳中毒(ACOP)患者的血清神经元特异性烯醇化酶(NSE)、髓鞘碱性蛋白(MBP)、Tau蛋白、多巴胺(DA)及中枢神经特异蛋白β(S100β)的水平变化,并分析ACOP后的脑损伤机制。方法选取2021年11月至2023年2月于达州市中心医院神经内科治疗的61例ACOP患者作为观察组,46例职业健康体检者作为对照组。采用ELISA法分别检测所有受试者的NSE、MBP、Tau蛋白、DA及S100β水平,并进行统计学分析。结果与对照组比较,观察组患者的血清NSE、MBP、Tau蛋白及S100β水平均显著升高(P<0.05),观察组DA水平与对照组比较,差异无统计学意义(P>0.05);经Pearson相关系数法分析结果显示,NSE与MBP水平呈正相关(r=0.211,P<0.05);DA与Tau、MBP、S100β水平均呈正相关(r=0.542、0.563、0.508,P<0.05);S100β与Tau、MBP水平均呈正相关(r=0.352、0.372,P<0.05)。结论ACOP患者血清中的NSE、MBP、S100β及Tau蛋白水平均呈高表达,这可能与ACOP患者脑损伤程度有关。

Objective To investigate the level changes of serum neuron specific enolase(NSE),myelin basic protein(MBP),Tau protein,dopamine(DA)and central nervous system specific proteinβ(S100β)in patients with brain injury after acute carbon monoxide poisoning(ACOP),and explore the mechanism of brain injury after ACOP.Methods Sixty-one patients with ACOP at the Department of Neurology in Dazhou Central Hospital from November 2021 to February 2023 were selected as observation group,and 46 individuals for occupational health examination were selected as control group.The levels of NSE,MBP,Tau protein,DA and S100βin all subjects were detected using Elisa assay,respectively,and statistically analyzed.Results When compared with control group,the levels of serum NSE,MBP,Tau protein and S100βwere significantly increased in observation group(P<0.05),but there was no statistically significant difference in DA level between observation and control groups(P>0.05).Pearson correlation coefficient analysis showed positive associations,including NSE with MBP(r=0.211,P<0.05),DA with Tau,MBP and S100β(r=0.542,0.563,0.508,P<0.05,respectively),S100βwith Tau and MBP(r=0.352,0.372,P<0.05,respectively).Conclusion The expression levels of NSE,MBP,S100β,and Tau protein are elevated in the serum of the patients after ACOP.It might be related to the degree of brain injury in the patients with ACOP.