参苓承气汤治疗腹腔内高压的作用机制探讨

To Explore the Mechanism of Shenling Chengqi Decoction(参苓承气汤)in the Treatment of lah Based on Network Pharmacology

目的 探讨参苓承气汤治疗腹腔内高压/腹腔间歇综合征的机制。方法 从TCMSP数据库获得参苓承气汤的成分和靶点。在DisGeNET、中国知网、PubMed等3个数据库获检索腹腔内高压/腹腔间歇综合征相关疾病靶点。数据处理获得药物与疾病的交集靶点。用Cytoscape 3.7.2软件构建“药物-成分-疾病”拓扑网络图。利用DAVID 6.8在线工具对交集靶点进行GO分析富集分析。在京都基因和基因组百科全书检索交集靶点的KRGG富集通路。结果 参苓承气汤中的6个关键成分通过5个关键靶点发挥治疗腹腔内高压/腹腔间歇综合征的作用。GO富集分析及KEGG富集分析显示,IL-6、NOS3、AHSA1、ALPI、F11R等5个关键靶基因富集在5个生物学过程、1个细胞组分、2个分子功能、72个KEGG通路。结论 参苓承气汤通过多靶点、多通路发挥治疗腹腔内高压/腹腔间歇综合征的作用。作用机制可能与多通路保护胃肠黏膜屏障、调控炎症反应等有关。

Objective To investigate the mechanism of the treatment of IAH/ACS by Shenling Chengqi Decoction(参苓承气汤).Methods The molecules and targets of Shenling Chengqi Decoction were obtained from TCMSP database.The disease targets related to IAH/ACS were retrieved from three databases including DisGeNET,CNKI.COM and PubMed.Data processing to ob-tain drug and disease intersection targets.Cytoscape 3.7.2 sofware was used to construct the topological network diagram of"drug-component-disease".DAVID 6.8 online tool was used for GO analysis enrichment analysis of intersection targets.KEGG enrichment pathways for intersection targets were searched in the Kyoto Encyclopedia of Genes and Genomes.Results GO enrich-ment analysis and KECG enrichment analysis showed that IL-6,NOS3,AHSA1,ALPI,F11R and other 5 key target genes of 6 key molecules of Shenling Chengqi Decoction were enriched in 5 biological processes,1 cell component,2 molecular functions and 72 KEGG pathways.Conclusion Shenling Chengqi Decoction play a role in the treatment of IAH/ACS through five key tar-gets.The mechanism may be related to multi-pathway protection of gastrointestinal mucosal barrier and regulation of inflammato-ryresponse.