泡型包虫病患者肝脏中蛋白基因产物9.5阳性神经纤维的检测及其分析

Detection and analysis of protein gene product 9.5 positive nerve fibers in liver of patients with alveolar echinococcosis

目的探索蛋白基因产物9.5(protein gene product 9.5,PGP9.5)在泡型包虫病(alveolar echinococcosis,AE)患者肝组织中病灶近端、远端的分布特征,明确泡型包虫病患者肝组织中病灶近端PGP9.5的阳性神经纤维密度与临床病理特征、生化指标之间的关系。方法选取2019年7月—2022年7月在新疆医科大学第一附属医院肝胆外科住院的59例泡型包虫病患者,收集患者病灶近端和远端的肝组织,并同期收集临床病理资料和生化指标信息。采用免疫组化方法检测59例泡型包虫病患者肝组织中病灶近端、远端的PGP9.5阳性神经纤维密度,分析泡型包虫病患者肝组织中病灶近端与远端PGP9.5阳性神经纤维密度的差异,进一步分析泡型包虫病患者病灶近端PGP9.5阳性神经纤维密度与临床病理特征、生化指标之间的关系。结果泡型包虫病患者肝组织内病灶近端的神经增多,且主要分布在病灶包裹的纤维膜外层,在病灶远端神经未见明显异常。泡型包虫病患者肝组织中病灶近端PGP9.5的阳性神经纤维密度显著高于病灶远端的PGP9.5阳性神经纤维密度,差异有统计学意义(Z=-4.237,P<0.05)。泡型包虫病患者肝组织中病灶近端PGP9.5阳性神经纤维密度与肝脏体积增大相关(Z=-2.632,P<0.05)。结论泡型包虫病患者病灶近端肝脏组织中PGP9.5阳性神经纤维面积增大,提示PGP9.5阳性神经参与了AE致病过程,其具体作用亟待进一步研究。

Objective To explore the distribution characteristics of protein gene product 9.5(PGP9.5)in the proximal and distal lesions of liver tissue in patients with alveolar echinococcosis(AE),and to clarify the relationship between the positive nerve fiber density of PGP9.5 in the proximal lesion of liver tissue of patients with AE and clinical pathological features and biochemical indexes.Methods From July 2019 to July 2022,59 patients with AE who were hospitalized in the Department of Hepatobiliary Surgery of the First Affiliated Hospital of Xinjiang Medical University were selected,and their liver tissues at the proximal and distal ends of the lesion were collected,and their clinicopathological data and biochemical index information were collected at the same time.Immunohistochemical method was used to detect the density of PGP9.5 positive nerve fibers in the proximal and distal liver tissues of 59 patients with AE,and to analyze the difference between the density of PGP9.5 positive nerve fibers in the proximal and distal liver tissues of patients with AE,and to further analyze the relationship between the density of PGP9.5 positive nerve fibers in the proximal liver tissues of patients with AE and clinicopathological features and biochemical indexes.Results The nerves in the proximal lesion of the liver tissue in patients with AE increased,mainly distributed in the outer layer of the fibrous capsule enclosing the lesion,and no obvious abnormalities were observed in the distal nerves.The density of PGP9.5 positive nerve fibers in the liver tissue of patients with AE was significantly higher than that in the distal part of the lesion,with statistical significance(Z=-4.237,P<0.05).The density of PGP9.5 positive nerve fibers in the liver tissue of patients with AE was correlated with the increase of liver volume(Z=-2.632,P<0.05).Conclusions The area of PGP9.5 positive nerve fibers in the proximal liver tissue of patients with alveolar echinococcosis increases,suggesting that PGP9.5 positive nerve is involved in the pathogenesis of AE,and its specific role needs further study.