IL-33、IL-37、NLRP3及NK/DC比值与慢性乙型肝炎患者病情相关性研究

Study on the correlation between IL-33,IL-37,NLRP3,NK/DC ratio and the condition of patients with chronic hepatitis B

目的探讨与研究白介素-33(IL-33)、白介素-37(IL-37)、亮氨酸富集的核苷酸结合寡聚结构域-3(NLRP-3)及自然杀伤(NK)细胞/树突状细胞(DC)比值与慢性乙型肝炎(CHB)患者病情的相关性。方法研究时间为2020年2月—2022年9月,选择在本院诊治的86例CHB患者作为肝炎组,同期选择86名体格检查健康者作为对照组。检测2组血清IL-37、IL-33、NLRP3含量,并计算NK/DC比值。对所有入选者的血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBIL)含量进行检测并实施相关性分析。结果与对照组相比,肝炎组的血清ALT、TBIL、AST有的增高趋势(P<0.05),肝炎组的血清IL-33、IL-37、NLRP3含量更高(P<0.05),NK/DC比值下降(P<0.05)。在肝炎组中,Pearson分析显示IL-33、IL-37、NLRP3、NK/DC比值与ALT、TBIL、AST均存在相关性(P<0.05)。在肝炎组中,ROC曲线分析显示IL-33、IL-37、NLRP3、NK/DC比值预测CHB病情的曲线下面积为0.705(95%CI:0.404~1.123)、0.690(95%CI:0.372~1.057)、0.670(95%CI:0.378~1.043)、0.685(95%CI:0.415~1.107),联合检测预测病情的曲线下面积为0.895(95%CI:0.532~1.216),与单独检测相比,联合检测具有更高的特异度与灵敏度。结论CHB患者多伴随有血清IL-33、IL-37、NLRP3的高表达,并且NK/DC比值会降低,IL-33、IL-37、NLRP3及NK/DC比值与CHB患者病情存在相关性,联合检测对患者病情具有一定的预测性。

Objective To explore and study the correlation between interleukin-33(IL-33),interleukin-37(IL-37),leucine-enriched nucleotide-binding oligomeric domain(NLRP)-3,the ratio of natural killer(NK)cells/dendritic cells(DC)and the conditions of patients with chronic hepatitis B(CHB).Methods From February 2020 to September 2022,86 patients with CHB treated in our hospital were selected as hepatitis group,and 86 healthy patients were selected as control group during the same period.The contents of IL-37,IL-33 and NLRP3 in serum of the two groups were detected,and NK/DC ratio was calculated.Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST)and total bilirubin(TBIL)of all the selected subjects were detected and correlation analysis was carried out.Results Compared with the control group,the serum ALT,TBIL and AST in hepatitis group were significantly increased(P<0.05),the contents of IL-33,IL-37 and NLRP3 were higher(P<0.05),and the NK/DC ratio was significantly decreased(P<0.05).In the hepatitis group,Pearson analysis showed that IL-33,IL-37,NLRP3 and NK/DC ratios were correlated with ALT,TBIL and AST(P<0.05).In the hepatitis group,ROC curve analysis showed that the maximum areas under the curve of IL-33,IL-37,NLRP3 and NK/DC ratios were 0.705(95%CI:0.404-1.123),0.690(95%CI:0.372-1.057),0.670(95%CI:0.378-1.043)and 0.685(95%CI:0.415-1.107),and the maximum area under the curve of combined detection was 0.895(95%CI:0.532-1.216).Compared with single detection,combined detection had higher specificity and sensitivity.Conclusions The patients with CHB are often accompanied by the high expression of serum IL-33,IL-37 and NLRP3,and the NK/DC ratio will be significantly reduced.IL-33,IL-37,NLRP3 and NK/DC ratio are correlated with the condition of patients with CHB,and can also predict the condition of patients.