高强度间歇运动对自发性高血压大鼠肾脏肾素-血管紧张素系统的影响

The effects of high-intensity interval training on the renin-angiotensin system in spontaneously hypertensive rats

目的观察高强度间歇运动对自发性高血压大鼠(SHR)血压水平及肾功能的影响,并探讨肾脏肾素-血管紧张素系统(RAS)在其间的作用机制。方法采用随机数字表法将20只雄性SHR大鼠分为高血压安静组及高血压运动组,同时选取10只Wistar-Kyoto大鼠纳入正常血压组。正常血压组及高血压安静组大鼠均置于鼠笼内安静饲养,高血压运动组大鼠则给予8周高强度间歇运动干预。于末次运动结束后检测各组大鼠血压水平、肾功能、肾脏一氧化氮(NO)和白细胞介素-6(IL-6)含量以及肾脏血管紧张素转换酶(ACE)、ACE2、血管紧张素1型受体(AT1R)、AT2R和Mas受体(MasR)蛋白表达量。结果与正常血压组比较,高血压安静组大鼠血压升高(P<0.05),肾功能减退(P<0.05),肾脏NO含量减少(P<0.05),IL-6含量升高(P<0.05),ACE和AT1R蛋白表达以及AT1R/AT2R比值升高(P<0.05),ACE2、AT2R及MasR蛋白表达下降(P<0.05);与高血压安静组比较,高血压运动组大鼠血压明显下降(P<0.05),肾功能改善(P<0.05),肾脏NO含量升高(P<0.05),IL-6含量降低(P<0.05),ACE和AT1R蛋白表达以及AT1R/AT2R比值下降(P<0.05),ACE2、AT2R及MasR蛋白表达显著上调(P<0.05)。结论8周高强度间歇运动干预能通过调控肾脏RAS轴对SHR大鼠肾脏发挥保护作用。

Objective To observe any effect of high-intensity interval training on the blood pressure and renal function of Wistar-Kyoto rats modeling spontaneous hypertension(SHR)and to explore the mechanism of the renal renin-angiotensin system′s(RAS′s)role in this process.Methods Twenty male SHR were randomly divided into a sedentary group and an exercise group,each of 10.Another 10 Wistar-Kyoto rats formed a normotensive control group.The rats in the normotensive and hypertensive sedentary groups were fed quietly in their cage,while the hypertensive exercise group performed high-intensity interval training for 8 weeks.After the last exercise,blood pressure,renal function,the kidney levels of nitric oxide and interleukin-6(IL-6)and the protein expressions of angiotensin converting enzyme(ACE),ACE2,angiotensin type 1 receptor(AT1R),AT2R and Mas receptor(MasR)were measured.Results Compared with the normotensive group,the hypertensive sedentary group showed a significant increase in average blood pressure,IL-6,ACE and AT1R protein and the ratio of AT1R to AT2R.There was a significant decrease in the renal function,the average NO level and the expression of ACE2,AT2R and MasR protein.That group also showed a significant decrease in blood pressure,IL-6,ACE and AT1R protein expression and the AT1R:AT2R ratio compared with the hypertensive sedentary group,but a significant increase in renal function,average NO content and the expression of ACE2,AT2R and MasR protein.Conclusion Eight weeks of high-intensity interval training has a protective effect on the kidneys by regulating the renin-angiotensin system,at least in rats.