摘要
目的 观察达雷妥尤单抗对凝聚胺技术检出IgG类抗D抗体的影响。方法 选取10份AB型RhD阳性、不规则抗体筛查阴性的血浆样本,将每份标本各分装2mL于3个试管中,分为3组,抗筛阴性对照组不做任何处理;加入IgG类抗D抗体的作为抗筛阳性对照组;加入达雷妥尤单抗(400mg/20mL)和IgG抗D试剂作为实验组。使用微柱凝胶卡法和凝聚胺法分别进行不规则抗体筛查试验,观察IgG抗D抗体的检出。结果 微柱凝胶卡法的抗体筛查试验结果显示:实验组(23.80±1.40)与阳性对照组(18.80±1.40)评分比较,差异有统计学意义(P <0.05);凝聚胺法的抗体筛查试验结果显示:实验组(14.80±2.53)与阳性对照组(14.80±2.53)评分比较,无统计学意义(P>0.05);且倍比稀释后实验组凝聚胺法对IgG抗D的检出下限同阳性对照组一致(1∶64)。结论 达雷妥尤单抗对凝聚胺法检出IgG类抗D抗体无影响,达雷妥尤单抗对微柱凝胶卡法检出IgG类抗D抗体有影响。
Objective To observe the effect of Daratumomab on detection of IgG anti-D antibody by polybrene test.Methods Ten plasma samples with AB RhD positive blood type and negative irregular antibody screening were selected,and each sample was divided into 3 groups by 2 mL in 3 tubes.Any substance was added in the antibody screening negative control group.IgG anti-D antibody was added in the antibody screening positive control group.Daretozumab(400 mg/20 mL)and IgG anti-D reagent were added in the experimental group.The irregular antibody screening test was performed by microcolumn gel card test and polybrene test to identify IgG anti-D antibody.Results There was significant difference between the experimental group(23.80±1.40)and the positive control group(18.80±1.40)by microcolumn gel card test(P<0.05).There was no significant difference between the experimental group(14.80±2.53)and the positive control group(14.80±2.53)by polybrene test(P>0.05).The lower limit of detection of IgG anti-D in the experimental group by polybrene test after dilution was the same result as that in the positive control group(1∶64).Conclusion Daretozumab has no effect on the detection of IgG anti D antibody by polybrene test,but has effect by the microcolumn gel card test.
作者
刘慧
卓海龙
骆群
LIU Hui;ZHUO Hailong;LUO Qun(The Fifth Medical Center of the General Hospital of the People's Liberation Army,Beijing 100071)
出处
《临床输血与检验》
CAS
2023年第5期625-630,共6页
Journal of Clinical Transfusion and Laboratory Medicine