基于泵浦-探测的瞬态成像:发展与前景

Transient imaging based on pump-probe technique:Advances and prospects

泵浦探测方法是研究超快过程广泛使用的基本方法之一.将泵浦探测方法与光学成像技术结合发展出的泵浦探测瞬态成像技术,可以实现高时间分辨、高空间分辨和高成像速度的微纳尺度成像和测量.在光学泵浦探测方法中,泵浦探测方法使用泵浦光激发待测物产生线性或非线性光学效应,使用探测光测量被激发的物质在时间和空间上的分布和迁移现象.根据作用原理的不同,现已开发出瞬态吸收显微镜、瞬态干涉散射显微镜和瞬态光热显微镜等泵浦探测瞬态成像技术.在材料领域,瞬态吸收显微镜和瞬态干涉散射显微镜可以在飞秒尺度研究超快物理过程,为材料的表征和改进提供指导.在生命科学领域,瞬态吸收和瞬态光热显微镜可以无损研究特定分子在细胞尺度下的分布情况,也可以测量亚细胞尺度传热相关的物理量及其空间分布的特性.本文简要介绍了泵浦探测方法用于微纳成像的研究进展,展望了其发展前景.

The pump-probe detection has been widely used to study ultrafast processes.The combination of pump-probe techniques and optical imaging enables the pump-probe transient imaging of micro/nano scale samples with high temporal and spatial resolution.In pump-probe detection,a pump beam is used to excite the sample,and a probe beam is used to measure the temporal and spatial distribution and transfer of the excited sample through linear or nonlinear optical effects.Different forms of transient pump-probe imaging methods have been developed,such as transient absorption microscope,transient interferometric scattering microscope and transient photothermal microscope,according to their principles.For material research,transient absorption microscopy,and transient interferometric scattering microscopy allow for the study of ultrafast physical processes at the femtosecond scale,providing guidance for the characterization and improvement of materials.For life science,transient absorption,and transient photothermal microscopy enable the study of the distribution of specific molecules at the cell scale in a non-destructive manner,and measure the distribution of physical quantities related to heat transfer in cells.This review briefly introduces the research advances and related prospects of pump-probe based transient imaging.