电针对CCH大鼠认知能力及海马p-JAK2、p-STAT3水平的影响

The effect of electroacupuncture on cognitive ability and hippocampus levels of p-JAK2 and p-STAT3 in CCH rats

目的探讨电针对慢性脑灌注不足(CCH)大鼠认知能力及海马p-JAK2、p-STAT3蛋白表达的影响。方法将40只健康清洁级雄性SD大鼠,随机分为为Sham组、Model组、Sham EA组、EA组。采用2-VO法制备CCH模型。通过激光多普勒血流仪检测各组大鼠不同时相rCBF情况,采用Morris水迷宫评价大鼠认知功能,Western Blot检测各组大鼠海马组织p-JAK2、p-STAT3蛋白表达。结果大鼠造模成功30 min后rCBF水平显著降低至基础血流的65%~75%之间(P<0.01);与Model组比较,EA组、Sham EA组大鼠rCBF升高(P<0.01),且随着干预时间的延长,EA组大鼠rCBF水平明显高于Sham EA组(P<0.05)。与Model组比较,EA组空间探索试验60 s平台象限停留时间明显延长(P<0.01),逃避潜伏期随着电针干预明显缩短(P<0.01)。与Sham EA组比较,EA组60 s平台象限停留时间明显延长(P<0.05),逃避潜伏期缩短(P<0.05)。与Model、Sham EA组比较,EA组海马p-JAK2、p-STAT3蛋白表达量下降(P<0.05)。结论电针百会和大椎可增加CCH大鼠rCBF、改善认知能力,可能与下调p-JAK2、p-STAT3蛋白表达有关。

Objective To investigate the effects of electroacupuncture(EA)on cognitive ability and the expression of p-JAK2 and p-STAT3 protein in hippocampus of rats with chronic cerebral hypoperfusion(CCH).Methods Forty male SD rats were randomly divided into the sham group,the model group,the sham EA group and the EA group.CCH model was copied by bilateral common carotid artery ligation method(2-VO),The regional cerebral blood flow(rCBF)of rats in different phases was detected by laser Doppler flowmetry,the cognitive function was evaluated by Morris water maze,and the expression of p-JAK2 and p-STAT3 protein in hippocampus was detected by Western blot.Results After 30 minutes of successful modeling,rCBF decreased significantly to 65~75%of basic blood flow(P<0.01).Compared with the model group,rCBF in the EA group and the sham EA group increased(P<0.01),and with the extension of intervention time,the rCBF level in the EA group was significantly higher than that in the sham EA group(P<0.05).Compared with the model group,the residence time in the 60 s platform quadrant of the EA group was significantly prolonged(P<0.01),and the escape latency was significantly shortened(P<0.01).Compared with the sham EA group,the residence time in 60 s platform quadrant in the EA group was significantly prolonged(P<0.05),and the escape latency was shortened(P<0.05).Compared with the model and the sham EA group,the expression of p-JAK2 and p-STAT3 protein in the EA group significantly decreased(P<0.05).Conclusion Electroacupuncture at Baihui and Dazhui can increase rCBF and improve cognitive ability in CCH rats,which may be related to down regulating the expression of p-JAK2 and p-STAT3 proteins.