花楸原花青素保存条件优化及其抗氧化性研究

Optimization of Preservation Conditions and Study on Antioxidant Properties of Rowan Proanthocyanidins

以黑果腺肋花楸中的原花青素保存率为考察指标,选取pH、保存温度、光照时间、恒温保存时间、空气暴露时间作为考察因素。通过三因素三水平的响应面分析法优化保存条件,可得到最佳保存条件:温度4℃,光照时间149.56 min,恒温保存时间16.18 min。在此条件下保存率可达到97.05%,接近预测值97.16%,相对误差为0.113%。同时,通过DPPH自由基清除能力的测定、羟自由基清除能力的测定和Fe^(3+)还原能力的测定3个试验对黑果腺肋花楸原花青素抗原性进行了研究。用蓝莓原花青素和抗坏血酸作为对照,黑果腺肋花楸原花青素总体抗氧化能力高于蓝莓原花青素和抗坏血酸。

The preservation rate of proanthocyanidins in P.nigra was taken as the investigation index,and the pH,storage temperature,natural light irradiation time,constant temperature storage time,and air exposure time was selected as the investigation factors.By optimizing the storage conditions by the three-factor three-level response surface analysis method,the best storage conditions could be obtained as temperature 4℃,natural light time 149.56 min,and constant temperature preservation time 16.18 min,Under these conditions,the preservation rate could reach 97.05%,which was close to the predicted value of 97.16%,and the relative error was 0.113%.At the same time,the antigenicity of proanthocyanidins of black fruit rib rowan was studied by three experiments of the determination of DPPH free radical scavenging capacity,the determination of hydroxyl radical scavenging capacity,and the determination of Fe^(3+) reduction ability.Using blueberry proanthocyanidins and ascorbic acid as controls,the overall antioxidant capacity of proanthocyanidins in black fruit rib rowan was greater than of blueberry proanthocyanidins and ascorbic acid.