从脾虚兔胃肠激素水平探讨高低频一指禅推法的补泻效应

Exploring Tonifying and Reducing Effect of High and Low Frequency One-Finger Meditation from Levels of Gastrointestinal Hormones in Rabbits with Spleen Deficiency

目的从脾虚新西兰兔血清胃泌素(gastrin,GAS)和胃窦组织一氧化氮(nitric oxide,NO)水平探讨高低频一指禅推法的补泻效应。方法取成年新西兰兔40只,随机分为空白组(K组)、模型组(P组)、一指禅推法低频组(A组)、一指禅推法高频组(B组)。A组和B组的手法治疗采用机械臂进行操作,治疗施加压力均为150 mN,A组频率为101~150次/min,B组频率为201~250次/min,选取中脘、双天枢,每穴推5 min,每次15 min,每天1次,连续10 d。治疗10 d后取家兔血清样本、胃窦组织测定GAS、NO水平,观察比较空白组(K组)、模型组(P组)、一指禅推法低频组(A组)、一指禅推法高频组(B组)4组家兔GAS、NO水平变化。结果血清GAS水平测定结果显示,K组血清GAS水平最低,P组血清GAS水平显著升高(P<0.05),A组和B组血清GAS水平较K组上升,较P组显著下降(P<0.05),且A组血清GAS水平下降较B组明显(P<0.05)。胃窦NO水平测定结果显示,K组胃窦NO水平最高,P组胃窦NO水平显著降低(P<0.05),A组和B组胃窦NO水平较K组下降,较P组显著上升(P<0.05),且A组胃窦NO水平上升较B组明显(P<0.05)。结论不同频率的一指禅推法对脾虚家兔血清GAS水平和胃窦NO水平的影响不同,101~150次/min低频率一指禅推可纠正脾虚家兔血清GAS水平及胃窦NO水平,使其趋近空白组正常水平,恢复胃肠功能,具有补的效应;201~250次/min高频率一指禅推治疗组家兔不仅未见血清GAS水平及胃窦NO水平的恢复,甚至呈恶化趋势,提示高频手法存在损害家兔胃肠功能的可能,具有泻的效应。

Objective To explore the tonifying and reducing effects of high and low frequency one-finger meditation from the levels of serum gastrin(GAS)and nitric oxide(NO)in gastric antrum tissue of New Zealand rabbits with spleen deficiency.Methods Forty adult New Zealand rabbits were randomly divided into blank group(group K),model group(group P),low frequency group(group A)and high frequency group(group B).The manipulator was used in group A and group B,and the pressure was 150 mn.The frequency of group A was 101-150 times/min,and that of group B was 201-250 times/min.Zhongwan(RN12)and Tianshu(ST25)(both side)were selected for 5 minutes per acupoint,15 minutes for each time,once a day for 10 consecutive days.After 10 days of treatment,the serum samples and gastric antrum tissues of rabbits were taken to determine the levels of GAS and NO.The changes of four groups were observed and compared:blank group(group K),model group(group P),low frequency group(group A)and high frequency group(group B).Results The results of serum GAS level measurement showed that the serum GAS level in group K was the lowest.Compared with that in group K,serum GAS level in P group was significantly increased(P<0.05).The serum GAS level in group A and B was increased compared with that in group K,and significantly decreased compared with that in group P(P<0.05).The level of NO in gastric antrum in group A was significantly higher than that in group B(P<0.05).Conclusion Different frequency of one-finger meditation has different effects on serum GAS level and gastric antrum NO level in spleen deficiency rabbits.Low frequency one-finger meditation can correct serum GAS level and gastric antrum NO level in spleen deficiency rabbits,make it close to the normal level of blank group,restore gastrointestinal function and have tonifying effect.There was no recovery of serum GAS level or gastric antrum NO level in rabbits treated with 201-250 bpm high-frequency one-finger meditation therapy,and even showed a trend of deterioration,suggesting that high-frequency manipulation may damage the gastrointestinal function of rabbits and have a purgative effect.