泽泻醇A对体外葡萄糖吸收的影响

Effect of Alisol A on Glucose Absorption in Vitro

目的:探讨泽泻醇A对人肝癌细胞(HepG2细胞)葡萄糖吸收活性的影响。方法:使用CCK-8法(Cell Counting Kit-8)测定HepG2细胞活力,利用2-NBDG(2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose)法测定细胞对葡萄糖的吸收能力,采用不同培养基培养HepG2细胞,通过流式细胞仪检测细胞荧光强度,从而考察不同浓度的泽泻醇A对HepG2细胞葡萄糖吸收的影响。结果:CCK-8实验结果表明,在1~10μmol/L范围内,泽泻醇A对HepG2细胞活力无明显影响。糖吸收实验结果表明泽泻醇A在不同程度上具有促进HepG2细胞对葡萄糖吸收的能力。结论:泽泻醇A可在一定程度上促进HepG2细胞对葡萄糖的吸收,提示泽泻醇A可能为泽泻降血糖的活性成分之一。

Objective To investigate the effect of Alisol A on the glucose absorption activity of human hepatoma cells(HepG2 cells).Methods Cell Counting Kit-8 method(CCK-8)was used to determine the viability of HepG2 cells,and 2-NBDGmethod was used to determine the glucose absorptioncapacity of cells.HepG2 cells were cultured in different media,and the fluorescence intensity of cells was detected by flow cytometry,so as to investigate the effects of different concentrations of alisol A on the glucose absorption of HepG2 cells.Results CCK-8 experiment showed that alisol A had no obvious effect on the viability of HepG2 cells in the range of 1~10μmol/L.The results of glucose absorption experiment showed that alisol A had the ability to promote the glucose absorption of HepG2 cells in different degrees.Conclusion Alisol A can promote the glucose absorption of HepG2 cells to a certain extent.It suggested that Alisol A may be one of the active ingredients of Alisma orientalis in hypoglycemic.