饮水锶对高血压小鼠血压及炎症功能的影响

Effect of strontium in drinking water on blood pressure and inflammatory function in hypertension mice

目的探讨饮水锶对高血压小鼠血压的影响及可能机制。方法按收缩压将100只ICR小鼠随机分为正常对照组(20只)和模型组(80只)。通过2 mg/mL N′-硝基-L-精氨酸甲酯盐酸盐饮水法(4周)建立小鼠高血压模型。将模型组80只高血压小鼠随机分为模型对照组(20只)及2.5、5.0和10.0 mg/L锶水组(各20只)。每周测定小鼠体重及血压水平,连续饮水干预10周后测定小鼠血清钠、钾、钙、镁、氯、一氧化氮、肾素、血管紧张素II、醛固酮及细胞间粘附分子-1含量,心脏中白细胞介素-6、白细胞介素-1β及肿瘤坏死因子含量。结果干预10周后,低、中、高锶水组小鼠收缩压分别为129.60±4.90 mmHg、127.33±6.35 mmHg和124.70±3.91 mmHg,显著低于模型对照组的141.84±5.34 mmHg(P<0.05)。高锶组小鼠舒张压为84.74±5.49 mmHg,显著低于模型对照组的92.21±10.08 mmHg。中锶水组小鼠血清钾、钙和镁含量分别为8.06±0.80 mmol/L、2.34±0.13 mmol/L和0.57±0.12 mmol/L,高锶水组小鼠的分别为9.59±0.58 mmol/L、2.37±0.17 mmol/L和0.58±0.09 mmol/L,显著高于正常对照组(分别为6.64±0.57 mmol/L、2.07±0.15 mmol/L、0.46±0.10 mmol/L)和模型对照组(分别为6.62±0.53 mmol/L、2.09±0.11 mmol/L、0.48±0.09 mmol/L),差异有统计学意义(P<0.05)。相较于模型对照组,高锶水组小鼠肾素(24.08±6.65 ng/mL vs.15.24±3.88 ng/mL)、血管紧张素II(263.30±61.66 pg/mL vs.203.31±54.95 pg/mL)、醛固酮(102.41±22.39 pg/mL vs.60.31±10.83 pg/mL)、细胞间粘附分子-1(367.17±120.08 ng/mL vs.224.45±46.86 ng/mL)、白细胞介素-6(5.90±0.66 ng/mL vs.3.88±1.08 ng/mL)、白细胞介素-1β(6.37±1.83 ng/mL vs.3.44±1.28 ng/mL)及肿瘤坏死因子(9.35±1.41 ng/mL vs.5.68±2.11 ng/mL)含量显著降低(P<0.05)。结论饮水锶可能通过调节内皮型一氧化氮合酶/一氧化氮通路,减少炎症因子的产生来降低高血压小鼠的血压水平。

OBJECTIVE To explore the effect of strontium in drinking water on blood pressure in hypertensive mice and its possible mechanism.METHODSEstablishment of mouse model of high blood pressure by drinking 2 mg/mL N′nitro-L-arginine methyl eater hydrochloride(L-NAME)for 4 weeks.One hundred ICR mice were randomly divided into normal control group(n=20)and model group(n=80)according to systolic blood pressure.Eighty hypertensive mice were randomly divided into model control group(n=20),2.5 mg/L strontium water group(n=20),5.0 mg/L strontium water group(n=20)and 10.0 mg/L strontium water group(n=20).The body weight and blood pressure of mice were measured every week.After 10 weeks,serum sodium(Na),potassium(K),calcium(Ca),magnesium(Mg),Chlorine(Cl),nitric oxide(NO),renin,angiotensin II(Ang II),aldosterone(ALD),endothelial nitric oxide synthase(eNOS),intercellular cell adhesion molecule-1(ICAM-1),heart interleukin-6(IL-6),interleukin-1 beta(IL-1β)and tumor necrosis factor-α(TNF-α)were determined.RESULTS After 10 weeks of intervention,the systolic blood pressure in the low,medium and high strontium water groups(129.60±4.90 mmHg vs.127.33±6.35 mmHg vs.124.70±3.91 mmHg)was significantly lower than that of the model control group(141.84±5.34 mmHg)(P<0.05).The diastolic blood pressure in the high strontium water group(84.74±5.49 mmHg)was significantly lower than that of the model control group(92.21±10.08 mmHg).The contents of serum potassium,calcium and magnesium in medium strontium gourp(8.06±0.80 mmol/L vs.2.34±0.13 mmol/L vs.0.57±0.12 mmol/L)and high strontium group(9.59±0.58 mmol/L vs.2.37±0.17 mmol/L vs.0.58±0.09 mmol/L)were significantly higher than those in normal control group(6.64±0.57 mmol/L vs.2.07±0.15 mmol/L vs.0.46±0.10 mmol/L)and model control group(6.62±0.53 mmol/L vs.2.09±0.11 mmol/L vs.0.48±0.09 mmol/L)(P<0.05).Compared with model control group,the contents of renin(24.08±6.65 ng/mL vs.15.24±3.88 ng/mL),AngII(263.30±61.66 pg/mL vs.203.31±54.95 pg/mL),ALD(102.41±22.39 pg/mL vs.60.31±10.83 pg/mL),ICAM-1(367.17±120.08 ng/mL vs.224.45±46.86 ng/mL),IL-6(5.90±0.66 ng/mL vs.3.88±1.08 ng/mL),IL-1β(6.37±1.83 ng/mL vs.3.44±1.28 ng/mL)and TNF-α(9.35±1.41 ng/mL vs.5.68±2.11 ng/mL)in high strontium group were significantly decreased(P<0.05).CONCLUSION Strontium can reduce the blood pressure of hypertensive mice by regulating the eNOS/NO pathway and reducing the production of inflammatory factors.