忍冬叶多糖提取工艺优化及抗菌活性分析

Optimization on Extraction of Polysaccharide from Honeysuckle(Lonicera japonica)Leaves and Its Antibacterial Activity

为明确忍冬叶多糖体外抑菌及保湿效果,以优化后的水提醇沉法提取忍冬叶多糖,采用预加菌液倾注平板-打孔法,验证忍冬叶多糖对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌的抑菌作用;并利用忍冬叶多糖制备忍冬多糖保湿霜,探究忍冬叶多糖的皮肤保湿效果。结果表明,优化后的提取方法所得的多糖含量为(17.29%±0.41%)。忍冬叶多糖对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌均有抑制作用,对大肠杆菌的抑制效果最佳,平均抑菌圈直径为(15.80±0.58) mm。皮肤水分测试结果表明,忍冬叶多糖具有良好的皮肤补水性及保湿性,具有潜在开发价值。以上研究结果优化了忍冬叶多糖提取工艺,为忍冬的深度开发及提高忍冬附加值奠定了基础。

In order to clarify the antibacterial and moisturizing effects of polysaccharide from honeysuckle leaves in vitro,the polysaccharide was extracted by the optimized water extraction and alcohol precipitation method in this experiment.The antibacterial experiments against Escherichia coli,Bacillus subtilis and Staphylococcus aureus were carried out by the pouring plate with pre-additive bacterial solution and punching method.The polysaccharide from Lonicera japonica leaves was used to prepare the polysaccharide moisturizing cream so as to explore its moisturizing effect.The results showed that the content of polysaccharide was(17.29%±0.41%)using the optimized extraction method.The polysaccharide of Lonicera japonica leaves had inhibitory effects on the 3 bacteria,among them,the inhibitory effect on Escherichia coli was the best,with an average diameter of(15.80±0.58)mm,which was highly sensitive.The skin moisture test showed that the polysaccharide from Lonicera japonica leaves had good skin rehydration and moisturizing properties,which had potential development value.The extraction process of polysaccharides from Lonicera japonica leaves,laying a foundation for the deep development of Lonicera japonica and continuously improving its added value.