摘要
目的:探索臭牡丹(Clerodendrum bungei Steud.,CBS)是否通过调控N-甲基-D-天冬氨酸(N-methyl-Daspartate,NMDA)受体缓解坐骨神经分支损伤(Spared nerve injury,SNI)诱导的大鼠神经病理性疼痛。方法:雄性SD大鼠18只,随机分成假手术组(Sham组)、SNI模型组(SNI组)、SNI+CBS治疗组(SNI+CBS组)。各组连续给予30 g·kg^(-1)CBS或等体积生理盐水治疗21 d。PCR方法检测背根神经节(Dorsal root ganglion,DRG)中NMDA受体亚基NR1、GluN2A、GluN2B、GluN3A mRNA表达;Western blot方法检测DRG中GluN2A、GluN2B和IL-10蛋白表达。结果:与Sham组相比,SNI模型大鼠DRG中NR1、GluN2B和GluN3A mRNA表达降低,差异有统计学意义(P<0.05);与SNI组相比,SNI+CBS治疗组NR1、GluN2B和GluN3A mRNA表达升高,各组GluN2A mRNA表达比较差异无统计学意义(P>0.05);与Sham组相比,SNI模型大鼠DRG中GluN2A和GluN2B蛋白水平升高,IL-10蛋白水平下降;与SNI组相比,SNI+CBS组GluN2A和GluN2B降低、IL-10升高,差异均有统计学意义(P<0.05);各组GluN2A的mRNA表达水平差异无统计学意义(P>0.05)。结论:臭牡丹可能通过抑制DRG炎症和调控NMDA受体缓解SNI诱导的大鼠神经病理性疼痛。
Objective:To explore whether Clerodendrum bungei Steud.(CBS)alleviate neuropathic pain of rats induced by spared nerve injury(SNI)through regulating N-methyl-D-aspartate(NMDA)receptors in rats.Methods:Eighteen male SD rats were randomly divided into sham-operation group,SNI group,SNI+CBS treatment group.The rats in each group were treated with 30 g·kg^(-1)CBS or the same volume of normal saline for 21 days.The mRNA expression of NMDA receptor subunits(NR1,GluN2A,GluN2B and GluN3A)in the dorsal root ganglion(DRG)was detected by Real-time quantification PCR.Western blot was used to evaluate protein levels of GluN2A,GluN2B and IL-10 in DRG.Results:Compared with sham group,mRNA expressions of NR1,GluN2B and GluN3A in DRG of SNI model rats were decreased,with statistical significance(P<0.05),which were increased in SNI+CBS group rats.Compared with sham group,the protein levels of GluN2A and GluN2B in DRG of SNI model rats were increased with no statistical significance(P>0.05),while the protein levels of IL-10 were decreased.Compared with SNI group,GluN2A and GluN2B were decreased and IL-10 was increased in SNI+CBS group,with statistical significance(P<0.05).The mRNA expression level of GluN2A in all the groups was with no statistical significance(P>0.05).Conclusion:CBS can alleviate inflammation and regulate NMDA receptor levels in the DRG in SNI,which may be involved in its inhibitory effect on neuropathic pain of rats.
作者
鞠艺楠
黄成炬
王安琪
温婷
黄诚
胡志苹
JU Yi-nan;HUANG Cheng-ju;WANG An-qi;WEN Ting;HUANG Cheng;HU Zhi-ping(The First Clinical Medical School,Gannan Medical University;School of Basic Medical,Gannan Medical University;The Neuroinflammation Lab of Ganzhou,Gannan Medical University,Ganzhou,Jiangxi 341000)
出处
《赣南医学院学报》
2023年第9期885-889,共5页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
基金
国家自然科学基金资助项目(30060213)
江西省教育厅科学技术研究项目(GJJ170863)
赣南医学院重点科研项目(ZD201704)
赣南医学院创新团队项目(TD201705,TD2021JC06)。